Effects of Low-Level Laser Therapy on M1-Related Cytokine Expression in Monocytes via Histone Modification
المؤلفون المشاركون
Liao, Wei-Ting
Chen, Chia-Hsin
Wang, Chau-Zen
Wang, Yan-Hsiung
Chen, Yi-Jen
Kuo, Chang-Hung
Kuo, Hsuan-Fu
Hung, Chih-Hsing
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-02-19
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Low-level laser therapy (LLLT) has been used in the treatment of radiotherapy-induced oral mucositis and allergic rhinitis.
However, the effects of LLLT on human monocyte polarization into M1 macrophages are unknown.
To evaluate the effects of LLLT on M1-related cytokine and chemokine production and elucidate the mechanism, the human monocyte cell line THP-1 was treated with different doses of LLLT.
The expression of M1-related cytokines and chemokines (CCL2, CXCL10, and TNF-α) was determined by ELISA and real-time PCR.
LLLT-associated histone modifications were examined by chromatin immunoprecipitation (ChIP) assays.
Mitochondrial involvement in the LLLT-induced M1-related cytokine expression was evaluated by quantitative real-time PCR.
Flow cytometry was used to detect the cell surface markers for monocyte polarization.
The results showed that LLLT (660 nm) significantly enhanced M1-related cytokine and chemokine expression in mRNA and protein levels.
Mitochondrial copy number and mRNA levels of complex I-V protein were increased by LLLT (1 J/cm2).
Activation of M1 polarization was concomitant with histone modification at TNF-α gene locus and IP-10 gene promoter area.
This study indicates that LLLT (660 nm) enhanced M1-related cytokine and chemokine expression via mitochondrial biogenesis and histone modification, which may be a potent immune-enhancing agent for the treatment of allergic diseases.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chen, Chia-Hsin& Wang, Chau-Zen& Wang, Yan-Hsiung& Liao, Wei-Ting& Chen, Yi-Jen& Kuo, Chang-Hung…[et al.]. 2014. Effects of Low-Level Laser Therapy on M1-Related Cytokine Expression in Monocytes via Histone Modification. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-1043686
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chen, Chia-Hsin…[et al.]. Effects of Low-Level Laser Therapy on M1-Related Cytokine Expression in Monocytes via Histone Modification. Mediators of Inflammation No. 2014 (2014), pp.1-13.
https://search.emarefa.net/detail/BIM-1043686
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chen, Chia-Hsin& Wang, Chau-Zen& Wang, Yan-Hsiung& Liao, Wei-Ting& Chen, Yi-Jen& Kuo, Chang-Hung…[et al.]. Effects of Low-Level Laser Therapy on M1-Related Cytokine Expression in Monocytes via Histone Modification. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-1043686
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1043686
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر