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G-Protein Coupled Receptor-Evoked Glutamate Exocytosis from Astrocytes: Role of Prostaglandins
المؤلفون المشاركون
Cali, Corrado
Lopatar, Jan
Petrelli, Francesco
Pucci, Luca
Bezzi, Paola
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-01-16
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Astrocytes are highly secretory cells, participating in rapid brain communication by releasing glutamate.
Recent evidences have suggested that this process is largely mediated by Ca2+-dependent regulated exocytosis of VGLUT-positive vesicles.
Here by taking advantage of VGLUT1-pHluorin and TIRF illumination, we characterized mechanisms of glutamate exocytosis evoked by endogenous transmitters (glutamate and ATP), which are known to stimulate Ca2+ elevations in astrocytes.
At first we characterized the VGLUT1-pHluorin expressing vesicles and found that VGLUT1-positive vesicles were a specific population of small synaptic-like microvesicles containing glutamate but which do not express VGLUT2.
Endogenous mediators evoked a burst of exocytosis through activation of G-protein coupled receptors.
Subsequent glutamate exocytosis was reduced by about 80% upon pharmacological blockade of the prostaglandin-forming enzyme, cyclooxygenase.
On the other hand, receptor stimulation was accompanied by extracellular release of prostaglandin E2 (PGE2).
Interestingly, administration of exogenous PGE2 produced per se rapid, store-dependent burst exocytosis of glutamatergic vesicles in astrocytes.
Finally, when PGE2-neutralizing antibody was added to cell medium, transmitter-evoked exocytosis was again significantly reduced (by about 50%).
Overall these data indicate that cyclooxygenase products are responsible for a major component of glutamate exocytosis in astrocytes and that large part of such component is sustained by autocrine/paracrine action of PGE2.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Cali, Corrado& Lopatar, Jan& Petrelli, Francesco& Pucci, Luca& Bezzi, Paola. 2014. G-Protein Coupled Receptor-Evoked Glutamate Exocytosis from Astrocytes: Role of Prostaglandins. Neural Plasticity،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1046670
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Cali, Corrado…[et al.]. G-Protein Coupled Receptor-Evoked Glutamate Exocytosis from Astrocytes: Role of Prostaglandins. Neural Plasticity No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1046670
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Cali, Corrado& Lopatar, Jan& Petrelli, Francesco& Pucci, Luca& Bezzi, Paola. G-Protein Coupled Receptor-Evoked Glutamate Exocytosis from Astrocytes: Role of Prostaglandins. Neural Plasticity. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1046670
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1046670
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
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