A Quantitative Method to Monitor Reactive Oxygen Species Production by Electron Paramagnetic Resonance in Physiological and Pathological Conditions
المؤلفون المشاركون
Gussoni, Maristella
Montorsi, Michela
Mrakic-Sposta, Simona
Vezzoli, Alessandra
Porcelli, Simone
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-10-12
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
The growing interest in the role of Reactive Oxygen Species (ROS) and in the assessment of oxidative stress in health and disease clashes with the lack of consensus on reliable quantitative noninvasive methods applicable.
The study aimed at demonstrating that a recently developed Electron Paramagnetic Resonance microinvasive method provides direct evidence of the “instantaneous” presence of ROS returning absolute concentration levels that correlate with “a posteriori” assays of ROS-induced damage by means of biomarkers.
The reliability of the choice to measure ROS production rate in human capillary blood rather than in plasma was tested (step I).
A significant ( P < 0.01 ) linear relationship between EPR data collected on capillary blood versus venous blood ( R 2 = 0.95 ), plasma ( R 2 = 0.82 ), and erythrocytes ( R 2 = 0.73 ) was found.
Then (step II) ROS production changes of various subjects’ categories, young versus old and healthy versus pathological at rest condition, were found significantly different (range 0.0001–0.05 P level).
The comparison of the results with antioxidant capacity and oxidative damage biomarkers concentrations showed that all changes indicating increased oxidative stress are directly related to ROS production increase.
Therefore, the adopted method may be an automated technique for a lot of routine in clinical trials.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Mrakic-Sposta, Simona& Gussoni, Maristella& Montorsi, Michela& Porcelli, Simone& Vezzoli, Alessandra. 2014. A Quantitative Method to Monitor Reactive Oxygen Species Production by Electron Paramagnetic Resonance in Physiological and Pathological Conditions. Oxidative Medicine and Cellular Longevity،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1047000
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Mrakic-Sposta, Simona…[et al.]. A Quantitative Method to Monitor Reactive Oxygen Species Production by Electron Paramagnetic Resonance in Physiological and Pathological Conditions. Oxidative Medicine and Cellular Longevity No. 2014 (Dec. 2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1047000
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Mrakic-Sposta, Simona& Gussoni, Maristella& Montorsi, Michela& Porcelli, Simone& Vezzoli, Alessandra. A Quantitative Method to Monitor Reactive Oxygen Species Production by Electron Paramagnetic Resonance in Physiological and Pathological Conditions. Oxidative Medicine and Cellular Longevity. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1047000
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1047000
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر