Cucurbitacin E Has Neuroprotective Properties and Autophagic Modulating Activities on Dopaminergic Neurons

المؤلفون المشاركون

Bournival, Julie
Arel-Dubeau, Anne-Marie
Tremblay, Cindy
Demers-Lamarche, Julie
Haskova, Pavlina
Attard, Everaldo
Germain, Marc
Martinoli, Maria-Grazia
Longpré, Fanny

المصدر

Oxidative Medicine and Cellular Longevity

العدد

المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-15، 15ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2014-12-09

دولة النشر

مصر

عدد الصفحات

15

التخصصات الرئيسية

الأحياء

الملخص EN

Natural molecules are under intensive study for their potential as preventive and/or adjuvant therapies for neurodegenerative disorders such as Parkinson’s disease (PD).

We evaluated the neuroprotective potential of cucurbitacin E (CuE), a tetracyclic triterpenoid phytosterol extracted from the Ecballium elaterium (Cucurbitaceae), using a known cellular model of PD, NGF-differentiated PC12.

In our postmitotic experimental paradigm, neuronal cells were treated with the parkinsonian toxin 1-methyl-4-phenylpyridinium (MPP+) to provoke significant cellular damage and apoptosis or with the potent N,N-diethyldithiocarbamate (DDC) to induce superoxide (O2•-) production, and CuE was administered prior to and during the neurotoxic treatment.

We measured cellular death and reactive oxygen species to evaluate the antioxidant and antiapoptotic properties of CuE.

In addition, we analyzed cellular macroautophagy, a bulk degradation process involving the lysosomal pathway.

CuE showed neuroprotective effects on MPP+-induced cell death.

However, CuE failed to rescue neuronal cells from oxidative stress induced by MPP+ or DDC.

Microscopy and western blot data show an intriguing involvement of CuE in maintaining lysosomal distribution and decreasing autophagy flux.

Altogether, these data indicate that CuE decreases neuronal death and autophagic flux in a postmitotic cellular model of PD.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Arel-Dubeau, Anne-Marie& Longpré, Fanny& Bournival, Julie& Tremblay, Cindy& Demers-Lamarche, Julie& Haskova, Pavlina…[et al.]. 2014. Cucurbitacin E Has Neuroprotective Properties and Autophagic Modulating Activities on Dopaminergic Neurons. Oxidative Medicine and Cellular Longevity،Vol. 2014, no. 2014, pp.1-15.
https://search.emarefa.net/detail/BIM-1047026

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Arel-Dubeau, Anne-Marie…[et al.]. Cucurbitacin E Has Neuroprotective Properties and Autophagic Modulating Activities on Dopaminergic Neurons. Oxidative Medicine and Cellular Longevity No. 2014 (Dec. 2014), pp.1-15.
https://search.emarefa.net/detail/BIM-1047026

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Arel-Dubeau, Anne-Marie& Longpré, Fanny& Bournival, Julie& Tremblay, Cindy& Demers-Lamarche, Julie& Haskova, Pavlina…[et al.]. Cucurbitacin E Has Neuroprotective Properties and Autophagic Modulating Activities on Dopaminergic Neurons. Oxidative Medicine and Cellular Longevity. 2014. Vol. 2014, no. 2014, pp.1-15.
https://search.emarefa.net/detail/BIM-1047026

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1047026