PRAK Interacts with DJ-1 and Prevents Oxidative Stress-Induced Cell Death
المؤلفون المشاركون
Tang, Jing
Liu, Jinghua
Li, Xue
Zhong, Yuyun
Zhong, Tianyu
Liu, Yawei
Wang, Jiang Huai
Jiang, Yong
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-10-14
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
As a core member of p38 MAPK signal transduction pathway, p38 regulated/activated kinase (PRAK) is activated by cellular stresses.
However, the function of PRAK and its downstream interacting partner remain undefined.
Using a yeast two-hybrid system, we identified DJ-1 as a potential PRAK interacting protein.
We further verified that DJ-1 bound to PRAK in vitro and in vivo and colocalized with PRAK in the nuclei of NIH3T3 cells.
Furthermore, following H2O2 stimulation the majority of endogenous DJ-1 in PRAK+/+ cells still remained in the nucleus, whereas most DJ-1 in PRAK−/− cells translocated from the nucleus into the cytoplasm, indicating that PRAK is essential for DJ-1 to localize in the nucleus.
In addition, PRAK-associated phosphorylation of DJ-1 was observed in vitro and in vivo of H2O2-challenged PRAK+/+ cells.
Cytoplasmic translocation of DJ-1 in H2O2-treated PRAK−/− cells lost its ability to sequester Daxx, a death protein, in the nucleus, and as a result, Daxx gained access to the cytoplasm and triggered cell death.
These data highlight that DJ-1 is the downstream interacting target for PRAK, and in response to oxidative stress PRAK may exert a cytoprotective effect by facilitating DJ-1 to sequester Daxx in the nucleus, thus preventing cell death.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Tang, Jing& Liu, Jinghua& Li, Xue& Zhong, Yuyun& Zhong, Tianyu& Liu, Yawei…[et al.]. 2014. PRAK Interacts with DJ-1 and Prevents Oxidative Stress-Induced Cell Death. Oxidative Medicine and Cellular Longevity،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-1047104
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Tang, Jing…[et al.]. PRAK Interacts with DJ-1 and Prevents Oxidative Stress-Induced Cell Death. Oxidative Medicine and Cellular Longevity No. 2014 (Dec. 2014), pp.1-13.
https://search.emarefa.net/detail/BIM-1047104
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Tang, Jing& Liu, Jinghua& Li, Xue& Zhong, Yuyun& Zhong, Tianyu& Liu, Yawei…[et al.]. PRAK Interacts with DJ-1 and Prevents Oxidative Stress-Induced Cell Death. Oxidative Medicine and Cellular Longevity. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-1047104
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1047104
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر