Magnetic Nanoparticle Based Nonviral MicroRNA Delivery into Freshly Isolated CD105+ hMSCs
المؤلفون المشاركون
David, Robert
Steinhoff, Gustav
Lux, Cornelia A.
Schade, Anna
Müller, Paula
Delyagina, Evgenya
Voronina, Natalia
Skorska, Anna
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-03-31
دولة النشر
مصر
عدد الصفحات
11
الملخص EN
Genetic modifications of bone marrow derived human mesenchymal stem cells (hMSCs) using microRNAs (miRs) may be used to improve their therapeutic potential and enable innovative strategies in tissue regeneration.
However, most of the studies use cultured hMSCs, although these can lose their stem cell characteristics during expansion.
Therefore, we aimed to develop a nonviral miR carrier based on polyethylenimine (PEI) bound to magnetic nanoparticles (MNPs) for efficient miR delivery in freshly isolated hMSCs.
MNP based transfection is preferable for genetic modifications in vivo due to improved selectivity, safety of delivery, and reduced side effects.
Thus, in this study different miR/PEI and miR/PEI/MNP complex formulations were tested in vitro for uptake efficiency and cytotoxicity with respect to the influence of an external magnetic field.
Afterwards, optimized magnetic complexes were selected and compared to commercially available magnetic vectors (Magnetofectamine, CombiMag).
We found that all tested transfection reagents had high miR uptake rates (yielded over 60%) and no significant cytotoxic effects.
Our work may become crucial for virus-free introduction of therapeutic miRs as well as other nucleic acids in vivo.
Moreover, in the field of targeted stem cell therapy nucleic acid delivery prior to transplantation may allowfor initial cell modulation in vitro.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Schade, Anna& Müller, Paula& Delyagina, Evgenya& Voronina, Natalia& Skorska, Anna& Lux, Cornelia A.…[et al.]. 2014. Magnetic Nanoparticle Based Nonviral MicroRNA Delivery into Freshly Isolated CD105+ hMSCs. Stem Cells International،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1047500
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Schade, Anna…[et al.]. Magnetic Nanoparticle Based Nonviral MicroRNA Delivery into Freshly Isolated CD105+ hMSCs. Stem Cells International No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1047500
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Schade, Anna& Müller, Paula& Delyagina, Evgenya& Voronina, Natalia& Skorska, Anna& Lux, Cornelia A.…[et al.]. Magnetic Nanoparticle Based Nonviral MicroRNA Delivery into Freshly Isolated CD105+ hMSCs. Stem Cells International. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1047500
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1047500
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر