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Effects of Toll-Like Receptors 3 and 4 in the Osteogenesis of Stem Cells
المؤلفون المشاركون
Qi, Chen
Xiaofeng, Xu
Xiaoguang, Wang
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-12-24
دولة النشر
مصر
عدد الصفحات
7
الملخص EN
Objective.
To investigate the effects of Toll-like receptors in stem cell osteogenesis.
Methods.
Bone marrow mesenchymal stem cells (BMSCs) were divided into the blank group, the TLR-3 activated group, and the TLR-4 activated group.
After 10 days’ osteogenic-promoting culture, expression of type I collagen and osteocalcin was determined by Western blot.
Osteoblasts (OBs) were also divided into three groups mentioned above.
Alkaline phosphatase (ALP) and alizarin red staining were performed after 10 days’ ossification-inducing culture.
The expression of β-catenin was investigated by Western blot.
Results.
Both the TLR-3 and TLR-4 activated groups had increased expression of type I collagen and osteocalcin; the effect of TLR-4 was stronger.
The intensity of alizarin red and ALP staining was strongest in the TLR-3 activated group and weakest in the TLR-4 activated group.
Activation of TLR-4 decreased the expression of β-catenin, whilst activation of TLR-3 did not affect the expression of β-catenin.
Discussion.
This study suggested that both TLR-3 and -4 promoted differentiation of BMSCs to OBs.
TLR-3 had an inducing effect on the ossification of OBs to osteocytes, whilst the effect of TLR-4 was the opposite because of its inhibitory effect on the Wnt signaling pathway.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Qi, Chen& Xiaofeng, Xu& Xiaoguang, Wang. 2014. Effects of Toll-Like Receptors 3 and 4 in the Osteogenesis of Stem Cells. Stem Cells International،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1047537
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Qi, Chen…[et al.]. Effects of Toll-Like Receptors 3 and 4 in the Osteogenesis of Stem Cells. Stem Cells International No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-1047537
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Qi, Chen& Xiaofeng, Xu& Xiaoguang, Wang. Effects of Toll-Like Receptors 3 and 4 in the Osteogenesis of Stem Cells. Stem Cells International. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1047537
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1047537
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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