Detection of β-Lactamases and Outer Membrane Porins among Klebsiella pneumoniae Strains Isolated in Iran
المؤلفون المشاركون
Fallah, Fatemeh
Hashemi, Ali
Erfanimanesh, Soroor
Alimehr, Shadi
Hamedani, Parastu
Goudarzi, Hossein
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-12-08
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
This descriptive study was accomplished on 83 K.
pneumoniae strains isolated from two hospitals in Tehran, Iran.
Antibiotic susceptibility tests were performed by disc diffusion and broth microdilution methods.
ESBLs, MBL, Amp-C, and KPC producing strains were detected by phenotypic confirmatory test, combination disk diffusion test (CDDT), Amp-C detection kit, and modified Hodge test, respectively.
OXA-48, NDM-1, and CTX-M-15 genes were detected by PCR and sequencing methods.
The outer membrane porins such as OmpK35 and OmpK36 were analysed by SDS-PAGE, PCR, and sequencing methods.
From 83 K.
pneumoniae isolates, 48 (57.5%), 3 (3.5%), 23 (28%), and 5 (6%) were ESBL, MBL, Amp-C, and KPC positive, respectively.
The CTX-M-15 gene was detected in 30 (62.5%) and OXA-48 gene was found in 2 (4.1%) of the 48 ESBL-producing isolates.
Two isolates harboured both OXA-48 and CTX-M-15; NDM-1 gene was not detected in this study.
Outer membrane porin, OmpK35, was detected in 30 (62.5%) of 48 ESBL-producing isolates while OmpK36 was found in 35 (72.91%) of 48 ESBL-producing isolates.
In this study, fosfomycin and tigecycline were more effective than other antibiotics.
The high prevalence of β-lactamase-producing K.
pneumoniae detected in this study is of great concern, which requires infection control measures including antibacterial management and identification of β-lactamases-producing isolates.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Hashemi, Ali& Fallah, Fatemeh& Erfanimanesh, Soroor& Hamedani, Parastu& Alimehr, Shadi& Goudarzi, Hossein. 2014. Detection of β-Lactamases and Outer Membrane Porins among Klebsiella pneumoniae Strains Isolated in Iran. Scientifica،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1047585
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Hashemi, Ali…[et al.]. Detection of β-Lactamases and Outer Membrane Porins among Klebsiella pneumoniae Strains Isolated in Iran. Scientifica No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-1047585
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Hashemi, Ali& Fallah, Fatemeh& Erfanimanesh, Soroor& Hamedani, Parastu& Alimehr, Shadi& Goudarzi, Hossein. Detection of β-Lactamases and Outer Membrane Porins among Klebsiella pneumoniae Strains Isolated in Iran. Scientifica. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1047585
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1047585
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر