Flow Cytometric Evaluation of T Cell Activation Markers after Cardiopulmonary Bypass
المؤلفون المشاركون
Mohr, Friedrich-Wilhelm
Dhein, Stefan
Dieterlen, Maja-Theresa
Bittner, Hartmuth B.
Tarnok, Attila
Garbade, Jens
Barten, Markus J.
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-02-06
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Background.
Cardiopulmonary bypass surgery (CPBS) is associated with an increased risk for infections or with subsequent organ dysfunction.
As T cell activation is a central mechanism during inflammatory processes, we developed an assay to evaluate T cell activation pathways in patients undergoing CPBS.
Methods.
Blood was obtained from eleven patients undergoing CPBS preoperatively, on postoperative day (POD)-3, and on POD-7 and was stimulated with different concentrations of Concanavalin A (ConA).
Cyclosporine and sirolimus, inhibiting different pathways of the T cell cycle, were added to blood ex vivo.
Expression of T cell activation markers CD25 and CD95 was analyzed by flow cytometry.
Results.
In untreated blood, expression of CD25 and CD95 significantly increased with higher ConA concentrations (P<0.05) and decreased for all ConA concentrations for both antigens over the study time (P<0.05).
Independently from the ConA concentration, inhibition of CD25 and CD95 expression was highest preoperatively for sirolimus and on POD-3 for cyclosporine.
At all time points, inhibition of CD25 and CD95 expression was significantly higher after cyclosporine compared to sirolimus treatment (P<0.001).
Conclusion.
Our results showed that different pathways of T cell activation are impaired after CPBS.
Such knowledge may offer the opportunity to identify patients at risk for postoperative complications.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Dieterlen, Maja-Theresa& Bittner, Hartmuth B.& Tarnok, Attila& Garbade, Jens& Dhein, Stefan& Mohr, Friedrich-Wilhelm…[et al.]. 2014. Flow Cytometric Evaluation of T Cell Activation Markers after Cardiopulmonary Bypass. Surgery Research and Practice،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1047667
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Dieterlen, Maja-Theresa…[et al.]. Flow Cytometric Evaluation of T Cell Activation Markers after Cardiopulmonary Bypass. Surgery Research and Practice No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-1047667
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Dieterlen, Maja-Theresa& Bittner, Hartmuth B.& Tarnok, Attila& Garbade, Jens& Dhein, Stefan& Mohr, Friedrich-Wilhelm…[et al.]. Flow Cytometric Evaluation of T Cell Activation Markers after Cardiopulmonary Bypass. Surgery Research and Practice. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1047667
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1047667
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر