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Mitigative Effect of Erythromycin on PMMA Challenged Preosteoblastic MC3T3-E1 Cells
المؤلفون المشاركون
Markel, David C.
Shen, Yi
Wang, Weili
Li, Xiaomiao
Ren, Weiping
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-06-03
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الطب البشري
تكنولوجيا المعلومات وعلم الحاسوب
الملخص EN
Background.
Aseptic loosening (AL) is a major complication of total joint replacement.
Recent approaches to limiting AL have focused on inhibiting periprosthetic inflammation and osteoclastogenesis.
Questions/Purposes.
The purpose of this study was to determine the effects of erythromycin (EM) on polymethylmethacrylate (PMMA) particle-challenged MC3T3 osteoblast precursor cells.
Methods.
MC3T3 cells were pretreated with EM (0–10 μg/mL) and then stimulated with PMMA (1 mg/mL).
Cell viability was evaluated by both a lactate dehydrogenase (LDH) release assay and cell counts.
Cell differentiation was determined by activity of alkaline phosphatase (ALP).
Gene expression was measured via real-time quantitative RT-PCR.
Results.
We found that exposure to PMMA particles reduced cellular viability and osteogenetic potential in MC3T3 cell line.
EM treatment mitigated the effects of PMMA particles on the proliferation, viability and differentiation of MC3T3 cells.
PMMA decreased the gene expression of Runx2, osterix and osteocalcin, which can be partially restored by EM treatment.
Furthermore, EM suppressed PMMA- induced increase of NF-κB gene expression.
Conclusions.
These data demonstrate that EM mitigates the effects of PMMA on MC3T3 cell viability and differentiation, in part through downregulation of NF-κB pathway.
EM appeared to represent an anabolic agent on MC3T3 cells challenged with PMMA particles.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Shen, Yi& Wang, Weili& Li, Xiaomiao& Markel, David C.& Ren, Weiping. 2014. Mitigative Effect of Erythromycin on PMMA Challenged Preosteoblastic MC3T3-E1 Cells. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1048334
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Shen, Yi…[et al.]. Mitigative Effect of Erythromycin on PMMA Challenged Preosteoblastic MC3T3-E1 Cells. The Scientific World Journal No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-1048334
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Shen, Yi& Wang, Weili& Li, Xiaomiao& Markel, David C.& Ren, Weiping. Mitigative Effect of Erythromycin on PMMA Challenged Preosteoblastic MC3T3-E1 Cells. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1048334
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1048334
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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