Derivation and Characterization of a ES-Like Cell Line from Indian Catfish Heteropneustes fossilis Blastulas

الملخص EN

A cell line designated as HFB-ES was established from blastula stage embryos of H.

fossilis (Singhi).

The embryonic cells were harvested and maintained in Leibovitz’s medium supplemented with 15% fetal bovine serum.

The cell line had been subcultured for more than 90 passages in a period of 24 months.

HFB-ES cells were able to grow at temperatures between 25 and 35°C with an optimum temperature of 28°C.

The growth rate of HFB-ES was proportional to FBS concentration, with optimum growth seen at 15% FBS concentration.

The originality of the cell line was confirmed by sequencing of cytochrome oxidase c subunit I (COI), cytochrome b gene, and microsatellite DNA profile.

Results of chromosome complements of HFB showed normal karyo-morphology with 56 (2 n ) diploid number of chromosomes after 40 passages which indicated that the developed cell line is chromosomally stable.

The pluripotency of HFB was demonstrated by alkaline phosphatase activity and Oct-4 gene expression.

Expression of GFP reporter gene was successful in HFB-ES.

These results indicated that HFB-ES could be utilized for future gene expression studies.