Molecular Characterization of Putative Virulence Determinants in Burkholderia pseudomallei
المؤلفون المشاركون
Chua, Kek Heng
Puah, Suat Moi
Wang, Jin Town
Pan, Yi Jiun
Puthucheary, Savithri
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-08-31
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الطب البشري
تكنولوجيا المعلومات وعلم الحاسوب
الملخص EN
The Gram-negative saprophyte Burkholderia pseudomallei is the causative agent of melioidosis, an infectious disease which is endemic in Southeast Asia and northern Australia.
This bacterium possesses many virulence factors which are thought to contribute to its survival and pathogenicity.
Using a virulent clinical isolate of B.
pseudomallei and an attenuated strain of the same B.
pseudomallei isolate, 6 genes BPSL2033, BP1026B_I2784, BP1026B_I2780, BURPS1106A_A0094, BURPS1106A_1131, and BURPS1710A_1419 were identified earlier by PCR-based subtractive hybridization.
These genes were extensively characterized at the molecular level, together with an additional gene BPSL3147 that had been identified by other investigators.
Through a reverse genetic approach, single-gene knockout mutants were successfully constructed by using site-specific insertion mutagenesis and were confirmed by PCR.
BPSL2033::Km and BURPS1710A_1419::Km mutants showed reduced rates of survival inside macrophage RAW 264.7 cells and also low levels of virulence in the nematode infection model.
BPSL2033::Km demonstrated weak statistical significance (P=0.049) at 8 hours after infection in macrophage infection study but this was not seen in BURPS1710A_1419::Km.
Nevertheless, complemented strains of both genes were able to partially restore the gene defects in both in vitro and in vivo studies, thus suggesting that they individually play a minor role in the virulence of B.
pseudomallei.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Puah, Suat Moi& Puthucheary, Savithri& Wang, Jin Town& Pan, Yi Jiun& Chua, Kek Heng. 2014. Molecular Characterization of Putative Virulence Determinants in Burkholderia pseudomallei. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1050238
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Puah, Suat Moi…[et al.]. Molecular Characterization of Putative Virulence Determinants in Burkholderia pseudomallei. The Scientific World Journal No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1050238
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Puah, Suat Moi& Puthucheary, Savithri& Wang, Jin Town& Pan, Yi Jiun& Chua, Kek Heng. Molecular Characterization of Putative Virulence Determinants in Burkholderia pseudomallei. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1050238
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1050238
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر