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Growth Phase-Dependent Proteomes of the Malaysian Isolated Lactococcus lactis Dairy Strain M4 Using Label-Free Qualitative Shotgun Proteomics Analysis
المؤلفون المشاركون
Abdul Murad, Abdul Munir
Illias, Rosli Md
Yap, Theresa Wan Chen
Rabu, Amir
Mahadi, Nor Muhammad
Abu Bakar, Farah Diba
Abdul Rahim, Raha
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-03-25
دولة النشر
مصر
عدد الصفحات
14
التخصصات الرئيسية
الطب البشري
تكنولوجيا المعلومات وعلم الحاسوب
الملخص EN
Lactococcus lactis is the most studied mesophilic fermentative lactic acid bacterium.
It is used extensively in the food industry and plays a pivotal role as a cell factory and also as vaccine delivery platforms.
The proteome of the Malaysian isolated L.
lactis M4 dairy strain, obtained from the milk of locally bred cows, was studied to elucidate the physiological changes occurring between the growth phases of this bacterium.
In this study, ultraperformance liquid chromatography nanoflow electrospray ionization tandem mass spectrometry (UPLC- nano-ESI-MSE) approach was used for qualitative proteomic analysis.
A total of 100 and 121 proteins were identified from the midexponential and early stationary growth phases, respectively, of the L.
lactis strain M4.
During the exponential phase, the most important reaction was the generation of sufficient energy, whereas, in the early stationary phase, the metabolic energy pathways decreased and the biosynthesis of proteins became more important.
Thus, the metabolism of the cells shifted from energy production in the exponential phase to the synthesis of macromolecules in the stationary phase.
The resultant proteomes are essential in providing an improved view of the cellular machinery of L.
lactis during the transition of growth phases and hence provide insight into various biotechnological applications.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yap, Theresa Wan Chen& Rabu, Amir& Abu Bakar, Farah Diba& Abdul Rahim, Raha& Mahadi, Nor Muhammad& Illias, Rosli Md…[et al.]. 2014. Growth Phase-Dependent Proteomes of the Malaysian Isolated Lactococcus lactis Dairy Strain M4 Using Label-Free Qualitative Shotgun Proteomics Analysis. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-1050469
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yap, Theresa Wan Chen…[et al.]. Growth Phase-Dependent Proteomes of the Malaysian Isolated Lactococcus lactis Dairy Strain M4 Using Label-Free Qualitative Shotgun Proteomics Analysis. The Scientific World Journal No. 2014 (2014), pp.1-14.
https://search.emarefa.net/detail/BIM-1050469
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yap, Theresa Wan Chen& Rabu, Amir& Abu Bakar, Farah Diba& Abdul Rahim, Raha& Mahadi, Nor Muhammad& Illias, Rosli Md…[et al.]. Growth Phase-Dependent Proteomes of the Malaysian Isolated Lactococcus lactis Dairy Strain M4 Using Label-Free Qualitative Shotgun Proteomics Analysis. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-1050469
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1050469
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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