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Highly Effective Ex Vivo Gene Manipulation to Study Kidney Development Using Self-Complementary Adenoassociated Viruses
المؤلفون المشاركون
Chen, Tie-Lin
Wang, Hong-Lian
Liu, Yun-Hong
Fang, Yin
Tan, Rui-Zhi
Zhou, Pu-Hui
Zhou, Qin
Lv, Xiao-Yan
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-07-14
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الطب البشري
تكنولوجيا المعلومات وعلم الحاسوب
الملخص EN
Background.
Ex vivo culture of intact embryonic kidney has become a powerful system for studying renal development.
However, few methods have been available for gene manipulation and have impeded the identification and investigation of genes in this developmental process.
Results.
Here we systemically compared eight different serotypes of pseudotyped self-complementary adenoassociated viruses (scAAVs) transduction in cultured embryonic kidney with a modified culture procedure.
We demonstrated that scAAV was highly effective in delivering genes into and expressing in compacted tissues.
scAAV serotypes 2 and 8 exhibited higher efficiency of transduction compared to others.
Expression kinetics assay revealed that scAAV can be used for gene manipulation at the study of UB branching and nephrogenesis.
Repressing WT1 in cultured kidney using shRNA impairs tubule formation.
We for the first time employed and validated scAAV as a gene delivery tool in cultured kidney.
Conclusions.
These findings are expected to expedite the use of the ex vivo embryonic kidney cultures for kidney development research.
For other ex vivo cultured organ models, scAAV could also be a promising tool for organogenesis study.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chen, Tie-Lin& Wang, Hong-Lian& Liu, Yun-Hong& Fang, Yin& Tan, Rui-Zhi& Zhou, Pu-Hui…[et al.]. 2014. Highly Effective Ex Vivo Gene Manipulation to Study Kidney Development Using Self-Complementary Adenoassociated Viruses. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1050593
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chen, Tie-Lin…[et al.]. Highly Effective Ex Vivo Gene Manipulation to Study Kidney Development Using Self-Complementary Adenoassociated Viruses. The Scientific World Journal No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1050593
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chen, Tie-Lin& Wang, Hong-Lian& Liu, Yun-Hong& Fang, Yin& Tan, Rui-Zhi& Zhou, Pu-Hui…[et al.]. Highly Effective Ex Vivo Gene Manipulation to Study Kidney Development Using Self-Complementary Adenoassociated Viruses. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1050593
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1050593
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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