A Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Cyprinid Herpesvirus 2 in Gibel Carp (Carassius auratus gibelio)‎

المؤلفون المشاركون

Zhang, Hui
Zeng, Lingbing
Fan, Yuding
Zhou, Yong
Xu, Jin
Ma, Jie

المصدر

The Scientific World Journal

العدد

المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-6، 6ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2014-01-19

دولة النشر

مصر

عدد الصفحات

6

التخصصات الرئيسية

الطب البشري
تكنولوجيا المعلومات وعلم الحاسوب

الملخص EN

A rapid and sensitive loop-mediated isothermal amplification (LAMP) assay for Cyprinid herpesvirus 2 (CyHV-2) detection in gibel carp was developed.

Following cloning and sequencing of the putative DNA helicase gene of CyHV-2 isolate from China, a set of four specific primers was designed based on the sequence.

The MgCl2 concentration and the reaction temperature were optimized to 6 mM, 64°C, respectively.

LAMP products were detected by visual inspection of a color change due to addition of SYBR Green I stain.

The specificity and sensitivity of the LAMP assay were determined.

No cross-reaction was observed with other fish DNA viruses including eel herpesvirus, koi herpesvirus, and Chinese giant salamander iridovirus.

The LAMP assay was found to be equally sensitive as nested PCR.

A comparative evaluation of 10 fish samples using LAMP and nested PCR assays showed an overall correlation in positive and negative results for CyHV-2.

These results indicate that the LAMP assay is simple, sensitive, and specific and has a great potential use for CyHV-2 detection in the laboratory and field.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Zhang, Hui& Zeng, Lingbing& Fan, Yuding& Zhou, Yong& Xu, Jin& Ma, Jie. 2014. A Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Cyprinid Herpesvirus 2 in Gibel Carp (Carassius auratus gibelio). The Scientific World Journal،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1050736

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Zhang, Hui…[et al.]. A Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Cyprinid Herpesvirus 2 in Gibel Carp (Carassius auratus gibelio). The Scientific World Journal No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-1050736

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Zhang, Hui& Zeng, Lingbing& Fan, Yuding& Zhou, Yong& Xu, Jin& Ma, Jie. A Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Cyprinid Herpesvirus 2 in Gibel Carp (Carassius auratus gibelio). The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1050736

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1050736