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Bioconversion of Airborne Methylamine by Immobilized Recombinant Amine Oxidase from the Thermotolerant Yeast Hansenula polymorpha
المؤلفون المشاركون
Zakalska, Oksana M.
Nitzan, Yeshayahu
Nisnevitch, Marina
Sigawi, Sasi
Zakalskiy, Andriy E.
Gonchar, Mykhailo V.
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-01-29
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الطب البشري
تكنولوجيا المعلومات وعلم الحاسوب
الملخص EN
Aliphatic amines, including methylamine, are air-pollutants, due to their intensive use in industry and the natural degradation of proteins, amino acids, and other nitrogen-containing compounds in biological samples.
It is necessary to develop systems for removal of methylamine from the air, since airborne methylamine has a negative effect on human health.
The primary amine oxidase (primary amine : oxygen oxidoreductase (deaminating) or amine oxidase, AMO; EC 1.4.3.21), a copper-containing enzyme from the thermotolerant yeast Hansenula polymorpha which was overexpressed in baker’s yeast Saccharomyces cerevisiae, was tested for its ability to oxidize airborne methylamine.
A continuous fluidized bed bioreactor (CFBR) was designed to enable bioconversion of airborne methylamine by AMO immobilized in calcium alginate (CA) beads.
The results demonstrated that the bioreactor with immobilized AMO eliminates nearly 97% of the airborne methylamine.
However, the enzymatic activity of AMO causes formation of formaldehyde.
A two-step bioconversion process was therefore proposed.
In the first step, airborne methylamine was fed into a CFBR which contained immobilized AMO.
In the second step, the gas flow was passed through another CFBR, with alcohol oxidase from the yeast H.
polymorpha immobilized in CA, in order to decompose the formaldehyde formed in the first step.
The proposed system provided almost total elimination of the airborne methylamine and the formaldehyde.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Sigawi, Sasi& Nisnevitch, Marina& Zakalska, Oksana M.& Zakalskiy, Andriy E.& Nitzan, Yeshayahu& Gonchar, Mykhailo V.. 2014. Bioconversion of Airborne Methylamine by Immobilized Recombinant Amine Oxidase from the Thermotolerant Yeast Hansenula polymorpha. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1051510
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sigawi, Sasi…[et al.]. Bioconversion of Airborne Methylamine by Immobilized Recombinant Amine Oxidase from the Thermotolerant Yeast Hansenula polymorpha. The Scientific World Journal No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1051510
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Sigawi, Sasi& Nisnevitch, Marina& Zakalska, Oksana M.& Zakalskiy, Andriy E.& Nitzan, Yeshayahu& Gonchar, Mykhailo V.. Bioconversion of Airborne Methylamine by Immobilized Recombinant Amine Oxidase from the Thermotolerant Yeast Hansenula polymorpha. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1051510
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1051510
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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