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An Efficient Protocol for Deriving Liver Stem Cells from Neonatal Mice: Validating Its Differentiation Potential
المؤلفون المشاركون
Dhanasekaran, Sugapriya
Sithambaram, Devilakshmi
Govarthanan, Kavitha
Biswal, Bijesh Kumar
Verma, Rama S.
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-10-18
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
The success of liver regeneration depends on the availability of suitable cell types and their potential to differentiate into functional hepatocytes.
To identify the stem cells which have the ability to differentiate into hepatocytes, we used neonatal liver as source.
However, the current protocol for isolating stem cells from liver involves enzymes like collagenase, hyaluronidase exposed for longer duration which limits the success.
This results in the keen interest to develop an easy single step enzyme digestion protocol for isolating stem cells from liver for tissue engineering approaches.
Thus, the unlimited availability of cell type favors setting up the functional recovery of the damaged liver, ensuring ahead success towards treating liver diseases.
We attempted to isolate liver stem derived cells (LDSCs) from mouse neonatal liver using single step minimal exposure to enzyme followed by in vitro culturing.
The cells isolated were characterized for stem cell markers and subjected to lineage differentiation.
Further, LDSCs were induced to hepatocyte differentiation and validated with hepatocyte markers.
Finally, we developed a reproducible, efficient protocol for isolation of LDSCs with functional hepatocytes differentiation potential, which further can be used as in vitro model system for assessing drug toxicity assays in various preclinical trials.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Dhanasekaran, Sugapriya& Sithambaram, Devilakshmi& Govarthanan, Kavitha& Biswal, Bijesh Kumar& Verma, Rama S.. 2015. An Efficient Protocol for Deriving Liver Stem Cells from Neonatal Mice: Validating Its Differentiation Potential. Analytical Cellular Pathology،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1052343
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Dhanasekaran, Sugapriya…[et al.]. An Efficient Protocol for Deriving Liver Stem Cells from Neonatal Mice: Validating Its Differentiation Potential. Analytical Cellular Pathology No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1052343
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Dhanasekaran, Sugapriya& Sithambaram, Devilakshmi& Govarthanan, Kavitha& Biswal, Bijesh Kumar& Verma, Rama S.. An Efficient Protocol for Deriving Liver Stem Cells from Neonatal Mice: Validating Its Differentiation Potential. Analytical Cellular Pathology. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1052343
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1052343
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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