Isolation and Time Lapse Microscopy of Highly Pure Hepatic Stellate Cells
المؤلفون المشاركون
Heymann, Felix
Weiskirchen, Ralf
Bartneck, Matthias
Warzecha, Klaudia Theresa
Tag, Carmen Gabriele
Sauer-Lehnen, Sibille
Trautwein, Christian
Tacke, Frank
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-07-16
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Hepatic stellate cells (HSC) are the main effector cells for liver fibrosis.
We aimed at optimizing HSC isolation by an additional step of fluorescence-activated cell sorting (FACS) via a UV laser.
HSC were isolated from livers of healthy mice and animals subjected to experimental fibrosis.
HSC isolation by iohexol- (Nycodenz) based density centrifugation was compared to a method with subsequent FACS-based sorting.
We assessed cellular purity, viability, morphology, and functional properties like proliferation, migration, activation marker, and collagen expression.
FACS-augmented isolation resulted in a significantly increased purity of stellate cells (>99%) compared to iohexol-based density centrifugation (60–95%), primarily by excluding doublets of HSC and Kupffer cells (KC).
Importantly, this method is also applicable to young animals and mice with liver fibrosis.
Viability, migratory properties, and HSC transdifferentiation in vitro were preserved upon FACS-based isolation, as assessed using time lapse microscopy.
During maturation of HSC in culture, we did not observe HSC cell division using time lapse microscopy.
Strikingly, FACS-isolated, differentiated HSC showed very limited molecular and functional responses to LPS stimulation.
In conclusion, isolating HSC from mouse liver by additional FACS significantly increases cell purity by removing contaminations from other cell populations especially KC, without affecting HSC viability, migration, or differentiation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Bartneck, Matthias& Warzecha, Klaudia Theresa& Tag, Carmen Gabriele& Sauer-Lehnen, Sibille& Heymann, Felix& Trautwein, Christian…[et al.]. 2015. Isolation and Time Lapse Microscopy of Highly Pure Hepatic Stellate Cells. Analytical Cellular Pathology،Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1052353
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Bartneck, Matthias…[et al.]. Isolation and Time Lapse Microscopy of Highly Pure Hepatic Stellate Cells. Analytical Cellular Pathology No. 2015 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1052353
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Bartneck, Matthias& Warzecha, Klaudia Theresa& Tag, Carmen Gabriele& Sauer-Lehnen, Sibille& Heymann, Felix& Trautwein, Christian…[et al.]. Isolation and Time Lapse Microscopy of Highly Pure Hepatic Stellate Cells. Analytical Cellular Pathology. 2015. Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1052353
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1052353
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر