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Isolation and Molecular Identification of Auxotrophic Mutants to Develop a Genetic Manipulation System for the Haloarchaeon Natrinema sp. J7-2
المؤلفون المشاركون
Lv, Jie
Wang, Shuai
Wang, Yuchen
Huang, Yuping
Chen, Xiangdong
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-16، 16ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-05-21
دولة النشر
مصر
عدد الصفحات
16
التخصصات الرئيسية
الملخص EN
Our understanding of the genus Natrinema is presently limited due to the lack of available genetic tools.
Auxotrophic markers have been widely used to construct genetic systems in bacteria and eukaryotes and in some archaeal species.
Here, we isolated four auxotrophic mutants of Natrinema sp.
J7-2, via 1-methyl-3-nitro-1-nitroso-guanidin mutagenesis, and designated them as J7-2-1, J7-2-22, J7-2-26, and J7-2-52, respectively.
The mutant phenotypes were determined to be auxotrophic for leucine (J7-2-1), arginine (J7-2-22 and J7-2-52), and lysine (J7-2-26).
The complete genome and the biosynthetic pathways of amino acids in J7-2 identified that the auxotrophic phenotype of three mutants was due to gene mutations in leuB (J7-2-1), dapD (J7-2-26), and argC (J7-2-52).
These auxotrophic phenotypes were employed as selectable makers to establish a transformation method.
The transformation efficiencies were determined to be approximately 103 transformants per µg DNA.
And strains J7-2-1 and J7-2-26 were transformed into prototrophic strains with the wild type genomic DNA, amplified fragments of the corresponding genes, or the integrative plasmids carrying the corresponding genes.
Additionally, exogenous genes, bgaH or amyH gene, were expressed successfully in J7-2-1.
Thus, we have developed a genetic manipulation system for the Natrinema genus based on the isolated auxotrophic mutants of Natrinema sp.
J7-2.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Lv, Jie& Wang, Shuai& Wang, Yuchen& Huang, Yuping& Chen, Xiangdong. 2015. Isolation and Molecular Identification of Auxotrophic Mutants to Develop a Genetic Manipulation System for the Haloarchaeon Natrinema sp. J7-2. Archaea،Vol. 2015, no. 2015, pp.1-16.
https://search.emarefa.net/detail/BIM-1053928
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Lv, Jie…[et al.]. Isolation and Molecular Identification of Auxotrophic Mutants to Develop a Genetic Manipulation System for the Haloarchaeon Natrinema sp. J7-2. Archaea No. 2015 (2015), pp.1-16.
https://search.emarefa.net/detail/BIM-1053928
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Lv, Jie& Wang, Shuai& Wang, Yuchen& Huang, Yuping& Chen, Xiangdong. Isolation and Molecular Identification of Auxotrophic Mutants to Develop a Genetic Manipulation System for the Haloarchaeon Natrinema sp. J7-2. Archaea. 2015. Vol. 2015, no. 2015, pp.1-16.
https://search.emarefa.net/detail/BIM-1053928
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1053928
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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