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The Potential of GMP-Compliant Platelet Lysate to Induce a Permissive State for Cardiovascular Transdifferentiation in Human Mediastinal Adipose Tissue-Derived Mesenchymal Stem Cells
المؤلفون المشاركون
De Falco, Elena
Chimenti, Isotta
Ibrahim, Mohsen
Rendina, Erino A.
Peruzzi, Mariangela
Bordin, Antonella
Ponti, Donatella
Iudicone, Paola
Pierelli, Luca
Calogero, Antonella
Siciliano, Camilla
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-10-01
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Human adipose tissue-derived mesenchymal stem cells (ADMSCs) are considered eligible candidates for cardiovascular stem cell therapy applications due to their cardiac transdifferentiation potential and immunotolerance.
Over the years, the in vitro culture of ADMSCs by platelet lysate (PL), a hemoderivate containing numerous growth factors and cytokines derived from platelet pools, has allowed achieving a safe and reproducible methodology to obtain high cell yield prior to clinical administration.
Nevertheless, the biological properties of PL are still to be fully elucidated.
In this brief report we show the potential ability of PL to induce a permissive state of cardiac-like transdifferentiation and to cause epigenetic modifications.
RTPCR results indicate an upregulation of Cx43, SMA, c-kit, and Thy-1 confirmed by immunofluorescence staining, compared to standard cultures with foetal bovine serum.
Moreover, PL-cultured ADMSCs exhibit a remarkable increase of both acetylated histones 3 and 4, with a patient-dependent time trend, and methylation at lysine 9 on histone 3 preceding the acetylation.
Expression levels of p300 and SIRT-1, two major regulators of histone 3, are also upregulated after treatment with PL.
In conclusion, PL could unravel novel biological properties beyond its routine employment in noncardiac applications, providing new insights into the plasticity of human ADMSCs.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Siciliano, Camilla& Chimenti, Isotta& Bordin, Antonella& Ponti, Donatella& Iudicone, Paola& Peruzzi, Mariangela…[et al.]. 2015. The Potential of GMP-Compliant Platelet Lysate to Induce a Permissive State for Cardiovascular Transdifferentiation in Human Mediastinal Adipose Tissue-Derived Mesenchymal Stem Cells. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1054426
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Siciliano, Camilla…[et al.]. The Potential of GMP-Compliant Platelet Lysate to Induce a Permissive State for Cardiovascular Transdifferentiation in Human Mediastinal Adipose Tissue-Derived Mesenchymal Stem Cells. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1054426
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Siciliano, Camilla& Chimenti, Isotta& Bordin, Antonella& Ponti, Donatella& Iudicone, Paola& Peruzzi, Mariangela…[et al.]. The Potential of GMP-Compliant Platelet Lysate to Induce a Permissive State for Cardiovascular Transdifferentiation in Human Mediastinal Adipose Tissue-Derived Mesenchymal Stem Cells. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1054426
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1054426
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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