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Establishment of Hairy Root Cultures of Rhaponticum carthamoides (Willd.) Iljin for the Production of Biomass and Caffeic Acid Derivatives
المؤلفون المشاركون
Skała, Ewa
Kicel, Agnieszka
Olszewska, Monika A.
Kiss, Anna K.
Wysokińska, Halina
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-02-24
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
The aim of the study was to obtain transformed roots of Rhaponticum carthamoides and evaluate their phytochemical profile.
Hairy roots were induced from leaf explants by the transformation of Agrobacterium rhizogenes strains A4 and ATCC 15834.
The best response (43%) was achieved by infection with A4 strain.
The effects of different liquid media (WPM, B5, SH) with full and half-strength concentrations of macro- and micronutrients on biomass accumulation of the best grown hairy root line (RC3) at two different lighting conditions (light or dark) were investigated.
The highest biomass (93 g L−1 of the fresh weight after 35 days) was obtained in WPM medium under periodic light.
UPLC-PDA-ESI-MS3 and HPLC-PDA analyses of 80% aqueous methanol extracts from the obtained hairy roots revealed the presence of eleven caffeoylquinic acids and their derivatives and five flavonoid glycosides.
The production of caffeoylquinic acids and their derivatives was elevated in hairy roots grown in the light.
Only light-grown hairy roots demonstrated the capability for the biosynthesis of such flavonoid glycosides as quercetagetin, quercetin, luteolin, and patuletin hexosides.
Chlorogenic acid, 3,5-di-O-caffeoylquinic acid and a tentatively identified tricaffeoylquinic acid derivative were detected as the major compounds present in the transformed roots.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Skała, Ewa& Kicel, Agnieszka& Olszewska, Monika A.& Kiss, Anna K.& Wysokińska, Halina. 2015. Establishment of Hairy Root Cultures of Rhaponticum carthamoides (Willd.) Iljin for the Production of Biomass and Caffeic Acid Derivatives. BioMed Research International،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1054521
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Skała, Ewa…[et al.]. Establishment of Hairy Root Cultures of Rhaponticum carthamoides (Willd.) Iljin for the Production of Biomass and Caffeic Acid Derivatives. BioMed Research International No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1054521
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Skała, Ewa& Kicel, Agnieszka& Olszewska, Monika A.& Kiss, Anna K.& Wysokińska, Halina. Establishment of Hairy Root Cultures of Rhaponticum carthamoides (Willd.) Iljin for the Production of Biomass and Caffeic Acid Derivatives. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1054521
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1054521
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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