The Role and Mechanism of α-Klotho in the Calcification of Rat Aortic Vascular Smooth Muscle Cells
المؤلفون المشاركون
Chen, Tianlei
Mao, Huijuan
Chen, Cheng
Wu, Lin
Wang, Ningning
Zhao, Xiufen
Qian, Jun
Xing, Changying
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-11-02
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Objective.
To investigate the role and possible mechanism of α-Klotho in the calcification and the osteogenic transition of cultured VSMCs.
Methods.
VSMCs were cultured in vitro and divided into 5 groups, each using a different medium: (1) control; (2) β-GP; (3) β-GP + Klotho; (4) β-GP + LiCl; (5) β-GP + Klotho + LiCl.
Calcium deposits were visualized using Alizarin Red S staining.
The calcium concentrations were determined by the o-cresolphthalein complexone method.
BMP2, Runx2 and β-catenin levels were estimated by western blotting, and the level of α-SMA was determined by using immunofluorescence at day 12.
Results.
β-GP induced an increase in the expression of BMP2, Runx2, and β-catenin.
The calcium content increased, and the expression of α-SMA decreased.
Alizarin Red S staining was positive under the high phosphorus conditions.
BMP2, Runx2, and β-catenin levels and the calcium content decreased when the cells were cultured with rmKlotho; however, the levels of each were upregulated after treatment with the LiCl.
Conclusions.
Klotho can ameliorate the calcification and osteogenic transition of VSMCs induced by β-GP.
The mechanism of Klotho in preventing calcification in VSMCs may be partially mediated by the inhibition of the Wnt/β-catenin signaling pathway.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chen, Tianlei& Mao, Huijuan& Chen, Cheng& Wu, Lin& Wang, Ningning& Zhao, Xiufen…[et al.]. 2015. The Role and Mechanism of α-Klotho in the Calcification of Rat Aortic Vascular Smooth Muscle Cells. BioMed Research International،Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1054578
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chen, Tianlei…[et al.]. The Role and Mechanism of α-Klotho in the Calcification of Rat Aortic Vascular Smooth Muscle Cells. BioMed Research International No. 2015 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1054578
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chen, Tianlei& Mao, Huijuan& Chen, Cheng& Wu, Lin& Wang, Ningning& Zhao, Xiufen…[et al.]. The Role and Mechanism of α-Klotho in the Calcification of Rat Aortic Vascular Smooth Muscle Cells. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1054578
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1054578
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر