Synthesis, 68Ga-Radiolabeling, and Preliminary In Vivo Assessment of a Depsipeptide-Derived Compound as a Potential PETCT Infection Imaging Agent
المؤلفون المشاركون
Ebenhan, Thomas
Kruger, Hendrick Gert
Zeevaart, Jan Rijn
Sathekge, Mike Machaba
Mokaleng, Botshelo B.
Ramesh, Suhas
Govender, Thavendran
Parboosing, Raveen
Hazari, Puja P.
Marjanovic-Painter, Biljana
Mishra, Anil K.
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-01-28
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Noninvasive imaging is a powerful tool for early diagnosis and monitoring of various disease processes, such as infections.
An alarming shortage of infection-selective radiopharmaceuticals exists for overcoming the diagnostic limitations with unspecific tracers such as 67/68Ga-citrate or 18F-FDG.
We report here TBIA101, an antimicrobial peptide derivative that was conjugated to DOTA and radiolabeled with 68Ga for a subsequent in vitro assessment and in vivo infection imaging using Escherichia coli-bearing mice by targeting bacterial lipopolysaccharides with PET/CT.
Following DOTA-conjugation, the compound was verified for its cytotoxic and bacterial binding behaviour and compound stability, followed by 68Gallium-radiolabeling.
µPET/CT using 68Ga-DOTA-TBIA101 was employed to detect muscular E.
coli-infection in BALB/c mice, as warranted by the in vitro results.
68Ga-DOTA-TBIA101-PET detected E.
coli-infected muscle tissue (SUV = 1.3–2.4) > noninfected thighs ( P = 0.322 ) > forearm muscles ( P = 0.092 ) > background ( P = 0.021 ) in the same animal.
Normalization of the infected thigh muscle to reference tissue showed a ratio of 3.0 ± 0.8 and a ratio of 2.3 ± 0.6 compared to the identical healthy tissue.
The majority of the activity was cleared by renal excretion.
The latter findings warrant further preclinical imaging studies of greater depth, as the DOTA-conjugation did not compromise the TBIA101’s capacity as targeting vector.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Mokaleng, Botshelo B.& Ebenhan, Thomas& Ramesh, Suhas& Govender, Thavendran& Kruger, Hendrick Gert& Parboosing, Raveen…[et al.]. 2015. Synthesis, 68Ga-Radiolabeling, and Preliminary In Vivo Assessment of a Depsipeptide-Derived Compound as a Potential PETCT Infection Imaging Agent. BioMed Research International،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1054885
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Mokaleng, Botshelo B.…[et al.]. Synthesis, 68Ga-Radiolabeling, and Preliminary In Vivo Assessment of a Depsipeptide-Derived Compound as a Potential PETCT Infection Imaging Agent. BioMed Research International No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1054885
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Mokaleng, Botshelo B.& Ebenhan, Thomas& Ramesh, Suhas& Govender, Thavendran& Kruger, Hendrick Gert& Parboosing, Raveen…[et al.]. Synthesis, 68Ga-Radiolabeling, and Preliminary In Vivo Assessment of a Depsipeptide-Derived Compound as a Potential PETCT Infection Imaging Agent. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1054885
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1054885
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر