Role of B7-H4 siRNA in Proliferation, Migration, and Invasion of LOVO Colorectal Carcinoma Cell Line
المؤلفون المشاركون
Peng, Hai-xia
Wu, Wei-qi
Yang, Da-ming
Jing, Rong
Li, Ji
Zhou, Feng-li
Jin, Yun-fei
Wang, Sai-yu
Chu, Yi-min
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-05-21
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Objectives.
Colorectal cancer is one of the most common malignancies.
Recent studies investigated that B7-H4 is highly expressed in various cancers.
We aimed at exploring the effect of B7-H4 siRNA on proliferation, invasion, and migration of LOVO cells which expressed B7-H4 notably.
Design and Methods.
Colon adenocarcinoma dataset was downloaded from The Cancer Genome Atlas.
35 colorectal cancer patients admitted to Shanghai Tongren Hospital were enrolled in this study.
Cell proliferation and cell cycle distribution were identified by CCK8 and flow cytometry, respectively.
Transwell assay was performed to detect the invasion and migration of LOVO cells.
CXCL12/CXCR4 expression and JAK2/STAT3 phosphorylation were determined by real-time PCR and western blot.
Results.
B7-H4 expressed is elevated in colorectal cancer tissues than in the adjacent normal tissues.
B7-H4 siRNA effectively inhibited the proliferation at 24 h and 48 h, arrested cell cycle at G0/G1, and suppressed cell invasion and migration.
Gene set enrichment analysis showed that CXCL12/CXCR4 and JAK/STAT were correlative with the B7-H4 expression.
Additionally, CXCL12/CXCR4 expression and JAK2/STAT3 phosphorylation were reduced.
Conclusions.
B7-H4 siRNA can effectively inhibit proliferation, invasion, and migration of LOVO cells by targeting CXCL12/CXCR4 and JAK2/STAT3 signaling, which can serve as a new target for colorectal carcinoma treatment.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Peng, Hai-xia& Wu, Wei-qi& Yang, Da-ming& Jing, Rong& Li, Ji& Zhou, Feng-li…[et al.]. 2015. Role of B7-H4 siRNA in Proliferation, Migration, and Invasion of LOVO Colorectal Carcinoma Cell Line. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1055088
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Peng, Hai-xia…[et al.]. Role of B7-H4 siRNA in Proliferation, Migration, and Invasion of LOVO Colorectal Carcinoma Cell Line. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1055088
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Peng, Hai-xia& Wu, Wei-qi& Yang, Da-ming& Jing, Rong& Li, Ji& Zhou, Feng-li…[et al.]. Role of B7-H4 siRNA in Proliferation, Migration, and Invasion of LOVO Colorectal Carcinoma Cell Line. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1055088
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1055088
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر