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In Vitro Metacyclogenesis of Leishmania (Viannia) braziliensis and Leishmania (Leishmania) amazonensis Clinical Field Isolates, as Evaluated by Morphology, Complement Resistance, and Infectivity to Human Macrophages
المؤلفون المشاركون
Oliveira, Arthur Maynart Pereira
Ribeiro-Dias, Fátima
Dorta, Miriam Leandro
Pereira, Ledice Inacia de Araújo
da Silva, Ildefonso Alves
Morato, Camila Imai
Quixabeira, Valéria Bernadete Leite
Horta, Maria Fátima
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-15، 15ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-01-28
دولة النشر
مصر
عدد الصفحات
15
التخصصات الرئيسية
الملخص EN
This study was designed to assess in vitro metacyclogenesis of Leishmania (Viannia) braziliensis and Leishmania (Leishmania) amazonensis clinical field isolates obtained from patient lesions (L.
braziliensis IMG3 and PPS6m; L.
amazonensis MAB6).
Metacyclogenesis was evaluated by different criteria, namely, promastigote size (morphometric analysis and flow cytometry), surface modifications (loss of lectin or monoclonal antibody (mAb) binding, complement resistance), and infectivity to human macrophages.
Growth curves were similar for all parasites evaluated.
The various features analyzed were expressed in a high percentage of promastigotes at 6th and 10th days of culture and a low percentage at the 2nd day.
However, in most isolates, these features, considered as markers of metacyclogenesis, seemed to develop with different time courses, since the percentages of metacyclic forms detected with each technique were usually different.
Parasites from 6th or 10th day and those negatively selected with lectin or mAb similarly infected human macrophages.
From all isolates analyzed, L.
amazonensis PH8 and MAB6 showed the highest and the lowest levels of susceptibility, respectively, to leishmanicidal activity of IFN-γ/LPS-activated macrophages.
Our results showed that by using different techniques to evaluate different aspects of metacyclogenesis (morphological and biochemical modifications) different percentages of metacyclic promastigotes can be detected in each isolate culture.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
da Silva, Ildefonso Alves& Morato, Camila Imai& Quixabeira, Valéria Bernadete Leite& Pereira, Ledice Inacia de Araújo& Dorta, Miriam Leandro& Oliveira, Arthur Maynart Pereira…[et al.]. 2015. In Vitro Metacyclogenesis of Leishmania (Viannia) braziliensis and Leishmania (Leishmania) amazonensis Clinical Field Isolates, as Evaluated by Morphology, Complement Resistance, and Infectivity to Human Macrophages. BioMed Research International،Vol. 2015, no. 2015, pp.1-15.
https://search.emarefa.net/detail/BIM-1055303
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
da Silva, Ildefonso Alves…[et al.]. In Vitro Metacyclogenesis of Leishmania (Viannia) braziliensis and Leishmania (Leishmania) amazonensis Clinical Field Isolates, as Evaluated by Morphology, Complement Resistance, and Infectivity to Human Macrophages. BioMed Research International No. 2015 (2015), pp.1-15.
https://search.emarefa.net/detail/BIM-1055303
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
da Silva, Ildefonso Alves& Morato, Camila Imai& Quixabeira, Valéria Bernadete Leite& Pereira, Ledice Inacia de Araújo& Dorta, Miriam Leandro& Oliveira, Arthur Maynart Pereira…[et al.]. In Vitro Metacyclogenesis of Leishmania (Viannia) braziliensis and Leishmania (Leishmania) amazonensis Clinical Field Isolates, as Evaluated by Morphology, Complement Resistance, and Infectivity to Human Macrophages. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-15.
https://search.emarefa.net/detail/BIM-1055303
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1055303
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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