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Dengue Patients Exhibit Higher Levels of PrM and E Antibodies Than Their Asymptomatic Counterparts
المؤلفون المشاركون
Kumari Natkunam, Santha
Yeo, Adeline Syin Lian
Rathakrishnan, Anusyah
Wang, Seok Mui
Ponnampalavanar, Sasheela
Sathar, Jameela
Manikam, Rishya
Devi Sekaran, Shamala
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-03-01
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Dengue virus infection is a common tropical disease which often occurs without being detected.
These asymptomatic cases provide information in relation to the manifestation of immunological aspects.
In this study, we developed an ELISA method to compare neutralizing effects of dengue prM and E antibodies between dengue patients and their asymptomatic household members.
Recombinant D2 premembrane (prM) was constructed, cloned, and tested for antigenicity.
The recombinant protein was purified and tested with controls by using an indirect ELISA method.
Positive dengue serum samples with their asymptomatic pair were then carried out onto the developed ELISA.
In addition, commercially available recombinant envelope (E) protein was used to develop an ELISA which was tested with the same set of serum samples in the prM ELISA.
Asymptomatic individuals showed preexisting heterotypic neutralizing antibodies.
The recombinant prM was antigenically reactive in the developed ELISA.
Dengue patients had higher prM and E antibodies compared to their household members.
Our study highlights the neutralizing antibodies levels with respect to dengue prM and E between dengue patients and asymptomatic individuals.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yeo, Adeline Syin Lian& Rathakrishnan, Anusyah& Wang, Seok Mui& Ponnampalavanar, Sasheela& Manikam, Rishya& Sathar, Jameela…[et al.]. 2015. Dengue Patients Exhibit Higher Levels of PrM and E Antibodies Than Their Asymptomatic Counterparts. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1055428
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yeo, Adeline Syin Lian…[et al.]. Dengue Patients Exhibit Higher Levels of PrM and E Antibodies Than Their Asymptomatic Counterparts. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1055428
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yeo, Adeline Syin Lian& Rathakrishnan, Anusyah& Wang, Seok Mui& Ponnampalavanar, Sasheela& Manikam, Rishya& Sathar, Jameela…[et al.]. Dengue Patients Exhibit Higher Levels of PrM and E Antibodies Than Their Asymptomatic Counterparts. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1055428
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1055428
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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