ZnO Nanoparticles Treatment Induces Apoptosis by Increasing Intracellular ROS Levels in LTEP-a-2 Cells

المؤلفون المشاركون

Wang, Caixia
Hu, Xiaoke
Gao, Yan
Ji, Yinglu

المصدر

BioMed Research International

العدد

المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-9، 9ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2015-08-03

دولة النشر

مصر

عدد الصفحات

9

التخصصات الرئيسية

الطب البشري

الملخص EN

Owing to the wide use of novel nanoparticles (NPs) such as zinc oxide (ZnO) in all aspects of life, toxicological research on ZnO NPs is receiving increasing attention in these days.

In this study, the toxicity of ZnO NPs in a human pulmonary adenocarcinoma cell line LTEP-a-2 was tested in vitro.

Log-phase cells were exposed to different levels of ZnO NPs for hours, followed by colorimetric cell viability assay using tetrazolium salt and cell survival rate assay using trypan blue dye.

Cell morphological changes were observed by Giemsa staining and light microscopy.

Apoptosis was detected by using fluorescence microscopy and caspase-3 activity assay.

Both intracellular reactive oxygen species (ROS) and reduced glutathione (GSH) were examined by a microplate-reader method.

Results showed that ZnO NPs (≥0.01 μg/mL) significantly inhibited proliferation ( P < 0.05 ) and induced substantial apoptosis in LTEP-a-2 cells after 4 h of exposure.

The intracellular ROS level rose up to 30–40% corresponding to significant depletion (approximately 70–80%) in GSH content in LTEP-a-2 cells ( P < 0.05 ), suggesting that ZnO NPs induced apoptosis mainly through increased ROS production.

This study elucidates the toxicological mechanism of ZnO NPs in human pulmonary adenocarcinoma cells and provides reference data for application of nanomaterials in the environment.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Wang, Caixia& Hu, Xiaoke& Gao, Yan& Ji, Yinglu. 2015. ZnO Nanoparticles Treatment Induces Apoptosis by Increasing Intracellular ROS Levels in LTEP-a-2 Cells. BioMed Research International،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1055434

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Wang, Caixia…[et al.]. ZnO Nanoparticles Treatment Induces Apoptosis by Increasing Intracellular ROS Levels in LTEP-a-2 Cells. BioMed Research International No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1055434

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Wang, Caixia& Hu, Xiaoke& Gao, Yan& Ji, Yinglu. ZnO Nanoparticles Treatment Induces Apoptosis by Increasing Intracellular ROS Levels in LTEP-a-2 Cells. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1055434

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1055434