Revealing Glycoproteins in the Secretome of MCF-7 Human Breast Cancer Cells
المؤلفون المشاركون
Chen, Yeng
Gopinath, Subash C. B.
Hashim, Onn H.
Tan, Aik-Aun
Phang, Wai-Mei
Kiew, Lik Voon
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-06-17
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Breast cancer is one of the major issues in the field of oncology, reported with a higher prevalence rate in women worldwide.
In attempt to reveal the potential biomarkers for breast cancer, the findings of differentially glycosylated haptoglobin and osteonectin in previous study have drawn our attention towards glycoproteins of secretome from the MCF-7 cancer cell line.
In the present study, further analyses were performed on the medium of MCF-7 cells by subjecting it to two-dimensional analyses followed by image analysis in contrast to the medium of human mammary epithelial cells (HMEpC) as a negative control.
Carboxypeptidase A4 (CPA4), alpha-1-antitrypsin (AAT), haptoglobin (HP), and HSC70 were detected in the medium of MCF-7, while only CPA4 and osteonectin (ON) were detected in HMEpC medium.
In addition, CPA4 was detected as upregulated in the MCF-7 medium.
Further analysis by lectin showed that CPA4, AAT, HP, and HSC70 were secreted as N-glycan in the medium of MCF-7, with HP also showing differentially N-glycosylated isoforms.
For the HMEpC, only CPA4 was detected as N-glycan.
No O-glycan was detected in the medium of HMEpC but MCF-7 expressed O-glycosylated CPA4 and HSC70.
All these revealed that glycoproteins could be used as glycan-based biomarkers for the prognosis of breast cancer.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Tan, Aik-Aun& Phang, Wai-Mei& Gopinath, Subash C. B.& Hashim, Onn H.& Kiew, Lik Voon& Chen, Yeng. 2015. Revealing Glycoproteins in the Secretome of MCF-7 Human Breast Cancer Cells. BioMed Research International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1055516
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Tan, Aik-Aun…[et al.]. Revealing Glycoproteins in the Secretome of MCF-7 Human Breast Cancer Cells. BioMed Research International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1055516
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Tan, Aik-Aun& Phang, Wai-Mei& Gopinath, Subash C. B.& Hashim, Onn H.& Kiew, Lik Voon& Chen, Yeng. Revealing Glycoproteins in the Secretome of MCF-7 Human Breast Cancer Cells. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1055516
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1055516
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر