Key Proteins of Activating Cell Death Can Be Predicted through a Kainic Acid-Induced Excitotoxic Stress
المؤلفون المشاركون
Tsai, Hsiu-Ling
Chang, Sue-Joan
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-01-28
دولة النشر
مصر
عدد الصفحات
14
التخصصات الرئيسية
الملخص EN
Epilepsy is a major neurological disorder characterized by spontaneous seizures accompanied by neurophysiological changes.
Repeated seizures can damage the brain as neuronal death occurs.
A better understanding of the mechanisms of brain cell death could facilitate the discovery of novel treatments for neurological disorders such as epilepsy.
In this study, a model of kainic acid- (KA-) induced neuronal death was established to investigate the early protein markers associated with apoptotic cell death due to excitotoxic damage in the rat cortex.
The results indicated that KA induces both apoptotic and necrotic cell death in the cortex.
Incubation with high concentrations (5 and 500 μM, >75%) and low concentrations (0.5 pM: 95% and 50 nM: 8%) of KA for 180 min led to necrotic and apoptotic cell death, respectively.
Moreover, proteomic analysis using two-dimensional gel electrophoresis and mass spectrometry demonstrated that antiapoptotic proteins, including heat shock protein 70, 3-mercaptopyruvate sulfurtransferase, tubulin-B-5, and pyruvate dehydrogenase E1 component subunit beta, were significantly higher in apoptosis than in necrosis induced by KA.
Our findings provide direct evidence that several proteins are associated with apoptotic and necrotic cell death in excitotoxicity model.
The results indicate that these proteins can be apoptotic biomarkers from the early stages of cell death.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Tsai, Hsiu-Ling& Chang, Sue-Joan. 2015. Key Proteins of Activating Cell Death Can Be Predicted through a Kainic Acid-Induced Excitotoxic Stress. BioMed Research International،Vol. 2015, no. 2015, pp.1-14.
https://search.emarefa.net/detail/BIM-1055629
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Tsai, Hsiu-Ling& Chang, Sue-Joan. Key Proteins of Activating Cell Death Can Be Predicted through a Kainic Acid-Induced Excitotoxic Stress. BioMed Research International No. 2015 (2015), pp.1-14.
https://search.emarefa.net/detail/BIM-1055629
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Tsai, Hsiu-Ling& Chang, Sue-Joan. Key Proteins of Activating Cell Death Can Be Predicted through a Kainic Acid-Induced Excitotoxic Stress. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-14.
https://search.emarefa.net/detail/BIM-1055629
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1055629
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر