Lentivirus-Mediated siRNA Targeting ER-α Inhibits Tumorigenesis and Induces Apoptosis in Hepatocarcinoma Cells

الملخص EN

Background and Objectives.

Estrogen receptor-α (ER-α) plays important roles in hepatocarcinogenesis.

Recent studies have shown that ER-α could lead to cell cycle progression or inhibition of apoptosis.

To better understand the role of ER-α, RNA interference (RNAi) was used to inhibit ER-α expression in the human hepatocellular carcinoma (HCC) cells.

Methods.

Lentivirus-mediated ER-α small interfering RNA (siRNA) was transfected into HCC cells Hep3B.

ER-α expression was monitored by real-time polymerase chain reaction (PCR) and western blot.

Cell proliferation, apoptosis, and invasion were examined by methyl thiazol tetrazolium (MTT), flow cytometry (FCM), and invasion assay, respectively.

Results.

ER-α siRNA efficiently downregulated the expression of ER-α in Hep3B cells at both mRNA and protein levels in a time-dependent manner.

ER-α siRNA also inhibited cell proliferation and reduced cell invasion (compared with other groups, P < 0.05 , resp.).

Furthermore, knockdown of ER-α slowed down the cell population at S phase and increased the rate of apoptosis ( P < 0.05 , resp.).

Conclusion.

ER-α knockdown suppressed the growth of HCC cells.

Thus, ER-α may play a very important role in carcinogenesis of HCC and its knockdown may offer a new potential gene therapy approach for human liver cancer in the future.