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Macrocolonies (Granules) Formation as a Cause of False-Negative Results in the MGIT 960 System: Cause Analysis and Correlation with Mycobacterial Species
المؤلفون المشاركون
Huang, Hairong
Yu, Xia
Zhao, Liping
Jiang, Guanglu
Ma, Yifeng
Li, Weimin
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-5، 5ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-11-04
دولة النشر
مصر
عدد الصفحات
5
التخصصات الرئيسية
الملخص EN
Background.
The viable mycobacterial bacilli can sometimes form granules in the Mycobacterium Growth Indicator Tube (MGIT) to produce instrument-negative outcomes when BACTEC MGIT 960 culture is performed.
The cause of this phenomenon has never been analyzed.
Methods.
Thirty-one instrument-negative, granule presenting MGIT vials were collected for conducting acid-fast staining and also liquid and solid subculture.
Species identification and drug susceptibility test were performed with the recovered strains.
Cultivation test was done by inoculating small amount of bacilli into the MGIT vials.
Results.
Twenty-four and twenty-nine of the tested MGIT vials were smear and culture positive, respectively.
In total, 18, 4, and 7 of the cultivated strains were identified as Mycobacterium tuberculosis complex, M.
intracellulare, and M.
xenopi, respectively.
When a limited amount of bacilli was inoculated, the granule formation was observed for M.
xenopi strains in the MGIT system.
Conclusions.
The granules observed in the instrument-negative MGIT vials consisted of viable bacilli, which emphasized the need of visual inspection to increase recovery rate.
Limited bacterial load and specific species might be the cause of granule forming.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yu, Xia& Zhao, Liping& Jiang, Guanglu& Ma, Yifeng& Li, Weimin& Huang, Hairong. 2015. Macrocolonies (Granules) Formation as a Cause of False-Negative Results in the MGIT 960 System: Cause Analysis and Correlation with Mycobacterial Species. BioMed Research International،Vol. 2015, no. 2015, pp.1-5.
https://search.emarefa.net/detail/BIM-1055714
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yu, Xia…[et al.]. Macrocolonies (Granules) Formation as a Cause of False-Negative Results in the MGIT 960 System: Cause Analysis and Correlation with Mycobacterial Species. BioMed Research International No. 2015 (2015), pp.1-5.
https://search.emarefa.net/detail/BIM-1055714
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yu, Xia& Zhao, Liping& Jiang, Guanglu& Ma, Yifeng& Li, Weimin& Huang, Hairong. Macrocolonies (Granules) Formation as a Cause of False-Negative Results in the MGIT 960 System: Cause Analysis and Correlation with Mycobacterial Species. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-5.
https://search.emarefa.net/detail/BIM-1055714
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1055714
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