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Performances and Reliability of Bruker Microflex LT and VITEK MS MALDI-TOF Mass Spectrometry Systems for the Identification of Clinical Microorganisms
المؤلفون المشاركون
Bilecen, Kivanc
Yaman, Gorkem
Ciftci, Ugur
Laleli, Yahya Rauf
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-18، 18ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-12-17
دولة النشر
مصر
عدد الصفحات
18
التخصصات الرئيسية
الملخص EN
In clinical microbiology laboratories, routine microbial identification is mostly performed using culture based methodologies requiring 24 to 72 hours from culturing to identification.
Matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) technology has been established as a cost effective, reliable, and faster alternative identification platform.
In this study, we evaluated the reliability of the two available MALDI-TOF MS systems for their routine clinical level identification accuracy and efficiency in a clinical microbiology laboratory setting.
A total of 1,341 routine phenotypically identified clinical bacterial and fungal isolates were selected and simultaneously analyzed using VITEK MS (bioMérieux, France) and Microflex LT (Bruker Diagnostics, Germany) MALDI-TOF MS systems.
For any isolate that could not be identified with either of the systems and for any discordant result, 16S rDNA gene or ITS1/ITS2 sequencing was used.
VITEK MS and Microflex LT correctly identified 1,303 (97.17%) and 1,298 (96.79%) isolates to the species level, respectively.
In 114 (8.50%) isolates initial phenotypic identification was inaccurate.
Both systems showed a similar identification efficiency and workflow robustness, and they were twice as more accurate compared to routine phenotypic identification in our sample pool.
MALDITOF systems with their accuracy and robustness offer a good identification platform for routine clinical microbiology laboratories.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Bilecen, Kivanc& Yaman, Gorkem& Ciftci, Ugur& Laleli, Yahya Rauf. 2015. Performances and Reliability of Bruker Microflex LT and VITEK MS MALDI-TOF Mass Spectrometry Systems for the Identification of Clinical Microorganisms. BioMed Research International،Vol. 2015, no. 2015, pp.1-18.
https://search.emarefa.net/detail/BIM-1055787
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Bilecen, Kivanc…[et al.]. Performances and Reliability of Bruker Microflex LT and VITEK MS MALDI-TOF Mass Spectrometry Systems for the Identification of Clinical Microorganisms. BioMed Research International No. 2015 (2015), pp.1-18.
https://search.emarefa.net/detail/BIM-1055787
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Bilecen, Kivanc& Yaman, Gorkem& Ciftci, Ugur& Laleli, Yahya Rauf. Performances and Reliability of Bruker Microflex LT and VITEK MS MALDI-TOF Mass Spectrometry Systems for the Identification of Clinical Microorganisms. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-18.
https://search.emarefa.net/detail/BIM-1055787
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1055787
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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