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Insufficient Discriminatory Power of Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry Dendrograms to Determine the Clonality of Multi-Drug-Resistant Acinetobacter baumannii Isolates from an Intensive Care Unit
المؤلفون المشاركون
Rim, John Hoon
Hong, Sung Kuk
Park, Yongjung
Kim, MyungSook
Park, Eun Suk
D’Souza, Roshan
Lee, Kyungwon
Lee, Yangsoon
Yong, Dongeun
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-05-25
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
While pulsed-field gel electrophoresis (PFGE) is recognized as the gold standard method for clonality analysis, MALDI-TOF MS has recently been spotlighted as an alternative tool for species identification.
Herein, we compared the dendrograms of multi-drug-resistant (MDR) Acinetobacter baumannii isolates by using MALDI-TOF MS with those by using PFGE.
We used direct colony and protein extraction methods for MALDI-TOF MS dendrograms.
The isolates with identical PFGE patterns were grouped into different branches in MALDI-TOF MS dendrograms.
Among the isolates that were classified as very close isolates in MALDI-TOF MS dendrogram, PFGE band patterns visually showed complete differences.
We numeralized similarity among isolates by measuring distance levels.
The Spearman rank correlation coefficient values were 0.449 and 0.297 between MALDI-TOF MS dendrogram using direct colony and protein extraction method versus PFGE, respectively.
This study is the first paper focusing solely on the dendrogram function of MALDI-TOF MS compared with PFGE.
Although MALDI-TOF MS is a promising tool to identify species in a rapid manner, our results showed that MALDI-TOF MS dendrograms could not substitute PFGE for MDR Acinetobacter baumannii clonality analysis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Rim, John Hoon& Lee, Yangsoon& Hong, Sung Kuk& Park, Yongjung& Kim, MyungSook& D’Souza, Roshan…[et al.]. 2015. Insufficient Discriminatory Power of Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry Dendrograms to Determine the Clonality of Multi-Drug-Resistant Acinetobacter baumannii Isolates from an Intensive Care Unit. BioMed Research International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1055853
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Rim, John Hoon…[et al.]. Insufficient Discriminatory Power of Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry Dendrograms to Determine the Clonality of Multi-Drug-Resistant Acinetobacter baumannii Isolates from an Intensive Care Unit. BioMed Research International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1055853
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Rim, John Hoon& Lee, Yangsoon& Hong, Sung Kuk& Park, Yongjung& Kim, MyungSook& D’Souza, Roshan…[et al.]. Insufficient Discriminatory Power of Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry Dendrograms to Determine the Clonality of Multi-Drug-Resistant Acinetobacter baumannii Isolates from an Intensive Care Unit. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1055853
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1055853
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
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