Quantitative Image Analysis of Epithelial and Stromal Area in Histological Sections of Colorectal Cancer: An Emerging Diagnostic Tool
المؤلفون المشاركون
Rogojanu, Radu
Thiem, U.
Heindl, A.
Mesteri, I.
Smochina, C.
Bises, G.
Seewald, Alexander K.
Ellinger, Isabella
Jaeger, Walter
Thalhammer, Theresia
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-10-22
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
In colorectal cancer (CRC), an increase in the stromal (S) area with the reduction of the epithelial (E) parts has been suggested as an indication of tumor progression.
Therefore, an automated image method capable of discriminating E and S areas would allow an improved diagnosis.
Immunofluorescence staining was performed on paraffin-embedded sections from colorectal tumors (16 samples from patients with liver metastasis and 18 without).
Noncancerous tumor adjacent mucosa (n=5) and normal mucosa (n=4) were taken as controls.
Epithelial cells were identified by an anti-keratin 8 (K8) antibody.
Large tissue areas (5–63 mm2/slide) including tumor center, tumor front, and adjacent mucosa were scanned using an automated microscopy system (TissueFAXS).
With our newly developed algorithms, we showed that there is more K8-immunoreactive E in the tumor center than in tumor adjacent and normal mucosa.
Comparing patients with and without metastasis, the E/S ratio decreased by 20% in the tumor center and by 40% at tumor front in metastatic samples.
The reduction of E might be due to a more aggressive phenotype in metastasis patients.
The novel software allowed a detailed morphometric analysis of cancer tissue compartments as tools for objective quantitative measurements, reduced analysis time, and increased reproducibility of the data.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Rogojanu, Radu& Thalhammer, Theresia& Thiem, U.& Heindl, A.& Mesteri, I.& Seewald, Alexander K.…[et al.]. 2015. Quantitative Image Analysis of Epithelial and Stromal Area in Histological Sections of Colorectal Cancer: An Emerging Diagnostic Tool. BioMed Research International،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1055944
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Rogojanu, Radu…[et al.]. Quantitative Image Analysis of Epithelial and Stromal Area in Histological Sections of Colorectal Cancer: An Emerging Diagnostic Tool. BioMed Research International No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1055944
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Rogojanu, Radu& Thalhammer, Theresia& Thiem, U.& Heindl, A.& Mesteri, I.& Seewald, Alexander K.…[et al.]. Quantitative Image Analysis of Epithelial and Stromal Area in Histological Sections of Colorectal Cancer: An Emerging Diagnostic Tool. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1055944
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1055944
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر