Isolation, Culture, and Characterization of Chicken Cartilage StemProgenitor Cells
المؤلفون المشاركون
Ma, Y. H.
Guan, Weijun
Bai, Chunyu
Li, Lu
Li, Xianglong
Li, Xiangchen
Ji, Meng
Zhang, Shuang
Li, Junjie
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-08-16
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
A chondrocyte progenitor population isolated from the surface zone of articular cartilage has become a promising cell source for cell-based cartilage repair.
The cartilage-derived stem/progenitor cells are multipotent stem cells, which can differentiate into three cell types in vitro including adipocytes, osteoblasts, and chondrocytes.
Much work has been done on cartilage stem/progenitor cells (CSPCs) from people, horses, and cattle, but the relatively little literature has been published about these cells in chickens.
In our work, CSPCs were isolated from chicken embryos in incubated eggs for 20 days.
In order to inquire into the biological characteristics of chicken CSPCs, immunofluorescence, reverse transcription-polymerase chain reaction (RT-PCR), and flow cytometry were adopted to detect the characteristic surface markers of CSPCs.
Primary CSPCs were subcultured to passage 22 and, for purpose of knowing the change of cell numbers, we drew the growth curves.
Isolated CSPCs were induced to adipocytes, osteoblasts, and chondrocytes.
Our results suggest that we have identified and characterised a novel cartilage progenitor population resident in chicken articular cartilage and CSPCs isolated from chickens possess similar biological characteristics to those from other species, which will greatly benefit future cell-based cartilage repair therapies.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Li, Lu& Ma, Y. H.& Li, Xianglong& Li, Xiangchen& Bai, Chunyu& Ji, Meng…[et al.]. 2015. Isolation, Culture, and Characterization of Chicken Cartilage StemProgenitor Cells. BioMed Research International،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1056017
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Li, Lu…[et al.]. Isolation, Culture, and Characterization of Chicken Cartilage StemProgenitor Cells. BioMed Research International No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1056017
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Li, Lu& Ma, Y. H.& Li, Xianglong& Li, Xiangchen& Bai, Chunyu& Ji, Meng…[et al.]. Isolation, Culture, and Characterization of Chicken Cartilage StemProgenitor Cells. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1056017
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1056017
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر