![](/images/graphics-bg.png)
Role of TLR4-Mediated PI3KAKTGSK-3β Signaling Pathway in Apoptosis of Rat Hepatocytes
المؤلفون المشاركون
Zhang, Xian
Jiang, Daorong
Jiang, Wei
Zhao, Min
Gan, Jianhe
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-12-07
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
We investigated the mechanism of the Toll-like receptor 4- (TLR4-) mediated PI3K/AKT/GSK-3β signaling pathway in rat hepatocytes apoptosis induced by LPS.
The cultured rat hepatocytes were treated with LPS alone or first pretreated with TLR4 inhibitor, AKT inhibitor, and GSK-3β inhibitor, respectively, and then stimulated with the same dose of LPS.
Cell viability, cell apoptotic rate, and apoptosis morphology were assessed; the level of P- A K T S e r 473 , P-GSK- 3 β S e r 9 , and active Caspase-3 and the ratio of Bax/Bcl-2 were evaluated.
The results indicated that cell viability decreased, while cell apoptotic rate increased with time after LPS stimulation.
The expression of P- A K T S e r 473 and P-GSK- 3 β S e r 9 in the LPS group decreased compared with the control, while the level of active Caspase-3 and the ratio of Bax/Bcl-2 were significantly increased.
These effects were attenuated by pretreatment with CLI-095.
In addition, the apoptotic ratio decreased after pretreatment with LiCl but increased following pretreatment with LY294002.
The expression of P- A K T S e r 473 further decreased following pretreatment with LY294002 and the expression of P-GSK- 3 β S e r 9 increased following pretreatment with LiCl.
Moreover, pretreatment with CLI-095 weakened LPS-induced nuclear translocation of GSK-3β.
Our findings suggest that the TLR4-mediated PI3K/AKT/GSK-3β signaling pathway is present in rat hepatocytes and participates in apoptosis of BRL-3A cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhang, Xian& Jiang, Daorong& Jiang, Wei& Zhao, Min& Gan, Jianhe. 2015. Role of TLR4-Mediated PI3KAKTGSK-3β Signaling Pathway in Apoptosis of Rat Hepatocytes. BioMed Research International،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1056209
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhang, Xian…[et al.]. Role of TLR4-Mediated PI3KAKTGSK-3β Signaling Pathway in Apoptosis of Rat Hepatocytes. BioMed Research International No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1056209
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhang, Xian& Jiang, Daorong& Jiang, Wei& Zhao, Min& Gan, Jianhe. Role of TLR4-Mediated PI3KAKTGSK-3β Signaling Pathway in Apoptosis of Rat Hepatocytes. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1056209
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1056209
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)