Role of TLR4-Mediated PI3KAKTGSK-3β Signaling Pathway in Apoptosis of Rat Hepatocytes
المؤلفون المشاركون
Zhang, Xian
Jiang, Daorong
Jiang, Wei
Zhao, Min
Gan, Jianhe
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-12-07
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
We investigated the mechanism of the Toll-like receptor 4- (TLR4-) mediated PI3K/AKT/GSK-3β signaling pathway in rat hepatocytes apoptosis induced by LPS.
The cultured rat hepatocytes were treated with LPS alone or first pretreated with TLR4 inhibitor, AKT inhibitor, and GSK-3β inhibitor, respectively, and then stimulated with the same dose of LPS.
Cell viability, cell apoptotic rate, and apoptosis morphology were assessed; the level of P- A K T S e r 473 , P-GSK- 3 β S e r 9 , and active Caspase-3 and the ratio of Bax/Bcl-2 were evaluated.
The results indicated that cell viability decreased, while cell apoptotic rate increased with time after LPS stimulation.
The expression of P- A K T S e r 473 and P-GSK- 3 β S e r 9 in the LPS group decreased compared with the control, while the level of active Caspase-3 and the ratio of Bax/Bcl-2 were significantly increased.
These effects were attenuated by pretreatment with CLI-095.
In addition, the apoptotic ratio decreased after pretreatment with LiCl but increased following pretreatment with LY294002.
The expression of P- A K T S e r 473 further decreased following pretreatment with LY294002 and the expression of P-GSK- 3 β S e r 9 increased following pretreatment with LiCl.
Moreover, pretreatment with CLI-095 weakened LPS-induced nuclear translocation of GSK-3β.
Our findings suggest that the TLR4-mediated PI3K/AKT/GSK-3β signaling pathway is present in rat hepatocytes and participates in apoptosis of BRL-3A cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhang, Xian& Jiang, Daorong& Jiang, Wei& Zhao, Min& Gan, Jianhe. 2015. Role of TLR4-Mediated PI3KAKTGSK-3β Signaling Pathway in Apoptosis of Rat Hepatocytes. BioMed Research International،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1056209
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhang, Xian…[et al.]. Role of TLR4-Mediated PI3KAKTGSK-3β Signaling Pathway in Apoptosis of Rat Hepatocytes. BioMed Research International No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1056209
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhang, Xian& Jiang, Daorong& Jiang, Wei& Zhao, Min& Gan, Jianhe. Role of TLR4-Mediated PI3KAKTGSK-3β Signaling Pathway in Apoptosis of Rat Hepatocytes. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1056209
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1056209
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر