Endothelial Progenitor Cell Fraction Contained in Bone Marrow-Derived Mesenchymal Stem Cell Populations Impairs Osteogenic Differentiation
المؤلفون المشاركون
Verrier, Sophie
Loibl, Markus
Lara de Freitas, Rafael
Bittermann, Gido
Alini, Mauro
Duttenhoefer, Fabian
Richards, R. Geoff
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-09-27
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
In bone tissue engineering (TE) endothelial cell-osteoblast cocultures are known to induce synergies of cell differentiation and activity.
Bone marrow mononucleated cells (BMCs) are a rich source of mesenchymal stem cells (MSCs) able to develop an osteogenic phenotype.
Endothelial progenitor cells (EPCs) are also present within BMC.
In this study we investigate the effect of EPCs present in the BMC population on MSCs osteogenic differentiation.
Human BMCs were isolated and separated into two populations.
The MSC population was selected through plastic adhesion capacity.
EPCs (CD34+ and CD133+) were removed from the BMC population and the resulting population was named depleted MSCs.
Both populations were cultured over 28 days in osteogenic medium (Dex+) or medium containing platelet lysate (PL).
MSC population grew faster than depleted MSCs in both media, and PL containing medium accelerated the proliferation for both populations.
Cell differentiation was much higher in Dex+ medium in both cases.
Real-time RT-PCR revealed upregulation of osteogenic marker genes in depleted MSCs.
Higher values of ALP activity and matrix mineralization analyses confirmed these results.
Our study advocates that absence of EPCs in the MSC population enables higher osteogenic gene expression and matrix mineralization and therefore may lead to advanced bone neoformation necessary for TE constructs.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Duttenhoefer, Fabian& Lara de Freitas, Rafael& Loibl, Markus& Bittermann, Gido& Richards, R. Geoff& Alini, Mauro…[et al.]. 2015. Endothelial Progenitor Cell Fraction Contained in Bone Marrow-Derived Mesenchymal Stem Cell Populations Impairs Osteogenic Differentiation. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1056321
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Duttenhoefer, Fabian…[et al.]. Endothelial Progenitor Cell Fraction Contained in Bone Marrow-Derived Mesenchymal Stem Cell Populations Impairs Osteogenic Differentiation. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1056321
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Duttenhoefer, Fabian& Lara de Freitas, Rafael& Loibl, Markus& Bittermann, Gido& Richards, R. Geoff& Alini, Mauro…[et al.]. Endothelial Progenitor Cell Fraction Contained in Bone Marrow-Derived Mesenchymal Stem Cell Populations Impairs Osteogenic Differentiation. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1056321
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1056321
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر