Downregulation of miR-221 Inhibits Cell Migration and Invasion through Targeting Methyl-CpG Binding Domain Protein 2 in Human Oral Squamous Cell Carcinoma Cells
المؤلفون المشاركون
He, Shuqi
Lai, Renfa
Chen, Dan
Yan, Wangxiang
Zhang, Zhaoqiang
Liu, Zhiguo
Ding, Xueqiang
Chen, Yu
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-12-16
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Oral squamous cell carcinoma (OSCC), the most frequent of all oral cancers, is a type of highly malignant tumors with a high capacity to invade locally and form distant metastases.
An increasing number of studies have shown that microRNAs (miRNAs) play an important role in regulating cancer metastasis and invasion.
In the present study, we detected the expression of miR-221 in two highly metastatic OSCC cell lines and two OSCC cell lines that are less metastatic using quantitative real-time PCR analysis (qRT-PCR).
The qRT-PCR results indicate that miR-221 is upregulated in highly metastatic OSCC cell lines.
Then, miR-221 expression was knocked down by transfection with miR-221 inhibitor, and UM1 cell migration and invasion were assessed using transwell migration and invasion assays.
The results indicate that inhibition of miR-221 suppressed migration and invasion of UM1 cells.
Furthermore, methyl-CpG binding domain protein 2 (MBD2) was identified as a direct target gene of miR-221.
Additionally, MBD2 silencing could partly reverse the effect of miR-221 on cell migration and invasion.
In conclusion, downregulation of miR-221 inhibits cell migration and invasion at least partially through targeting MBD2 in the human OSCC cell line UM1.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
He, Shuqi& Lai, Renfa& Chen, Dan& Yan, Wangxiang& Zhang, Zhaoqiang& Liu, Zhiguo…[et al.]. 2015. Downregulation of miR-221 Inhibits Cell Migration and Invasion through Targeting Methyl-CpG Binding Domain Protein 2 in Human Oral Squamous Cell Carcinoma Cells. BioMed Research International،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1056624
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
He, Shuqi…[et al.]. Downregulation of miR-221 Inhibits Cell Migration and Invasion through Targeting Methyl-CpG Binding Domain Protein 2 in Human Oral Squamous Cell Carcinoma Cells. BioMed Research International No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1056624
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
He, Shuqi& Lai, Renfa& Chen, Dan& Yan, Wangxiang& Zhang, Zhaoqiang& Liu, Zhiguo…[et al.]. Downregulation of miR-221 Inhibits Cell Migration and Invasion through Targeting Methyl-CpG Binding Domain Protein 2 in Human Oral Squamous Cell Carcinoma Cells. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1056624
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1056624
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر