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Cloning and Characterization of Spliced Variants of the Porcine G Protein Coupled Receptor 120
المؤلفون المشاركون
Peng, Jian
Wei, Hongkui
Song, Tongxing
Peng, Jie
Ren, Jiao
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-05-17
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
The polyunsaturated fatty acids (PUFAs) receptor GPR120 exerts a significant impact on systemic nutrient homeostasis in human and rodents.
However, the porcine GPR120 (pGPR120) has not been well characterized.
In the current study, we found that pGPR120 had 3 spliced variants.
Transcript 1 encoded 362-amino acids (aa) wild type pGPR120-WT, which shared 88% homology with human short form GPR120.
Transcript 1 was the mainly expressed transcript of pGPR120.
It was expressed predominantly in ileum, jejunum, duodenum, spleen, and adipose.
Transcript 3 (coding 320-aa isoform) was detected in spleen, while the transcript 2 (coding 310-aa isoform) was only slightly expressed in spleen.
A selective agonist for human GPR120 (TUG-891) and PUFAs activated SRE-luc and NFAT-luc reporter in HEK293T cells transfected with construct for pGPR120-WT but not pGPR120-V2.
However, 320-aa isoform was not a dominant negative isoform.
The extracellular signal-regulated kinase 1/2 (ERK1/2) phosphorylation levels in cells transfected with construct for pGPR120-WT were well activated by PUFAs, especially n-3 PUFA.
These results showed that although pGPR120 had 3 transcripts, transcript 1 which encoded pGPR120-WT was the mainly expressed transcript.
TUG-891 and PUFAs, especially n-3 PUFA, well activated pGPR120-WT.
The current study contributed to dissecting the molecular regulation mechanisms of n-3 PUFA in pigs.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Song, Tongxing& Peng, Jie& Ren, Jiao& Wei, Hongkui& Peng, Jian. 2015. Cloning and Characterization of Spliced Variants of the Porcine G Protein Coupled Receptor 120. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1056829
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Song, Tongxing…[et al.]. Cloning and Characterization of Spliced Variants of the Porcine G Protein Coupled Receptor 120. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1056829
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Song, Tongxing& Peng, Jie& Ren, Jiao& Wei, Hongkui& Peng, Jian. Cloning and Characterization of Spliced Variants of the Porcine G Protein Coupled Receptor 120. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1056829
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1056829
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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