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Biofilm-Forming Staphylococcus epidermidis Expressing Vancomycin Resistance Early after Adhesion to a Metal Surface
المؤلفون المشاركون
Koseki, Hironobu
Tomita, Masato
Osaki, Makoto
Chiba, Ko
Sakimura, Toshiyuki
Kajiyama, Shiro
Adachi, Shinji
Yonekura, Akihiko
Miyamoto, Takashi
Tsurumoto, T.
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-01-31
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
We investigated biofilm formation and time of vancomycin (VCM) resistance expression after adhesion to a metal surface in Staphylococcus epidermidis.
Biofilm-forming Staphylococcus epidermidis with a VCM MIC of 1 μg/mL was used.
The bacteria were made to adhere to a stainless steel washer and treated with VCM at different times and concentrations.
VCM was administered 0, 2, 4, and 8 hours after adhesion.
The amount of biofilm formed was evaluated based on the biofilm coverage rates (BCRs) before and after VCM administration, bacterial viability in biofilm was visually observed using the fluorescence staining method, and the viable bacterial count in biofilm was measured.
The VCM concentration required to decrease BCR significantly compared with that of VCM-untreated bacteria was 4 μg/mL, even in the 0 hr group.
In the 4 and 8 hr groups, VCM could not inhibit biofilm growth even at 1,024 μg/mL.
In the 8 hr group, viable bacteria remained in biofilm at a count of 104 CFU even at a high VCM concentration (1,024 μg/mL).
It was suggested that biofilm-forming Staphylococcus epidermidis expresses resistance to VCM early after adhesion to a metal surface.
Resistance increased over time after adhesion as the biofilm formed, and strong resistance was expressed 4–8 hours after adhesion.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Sakimura, Toshiyuki& Kajiyama, Shiro& Adachi, Shinji& Chiba, Ko& Yonekura, Akihiko& Tomita, Masato…[et al.]. 2015. Biofilm-Forming Staphylococcus epidermidis Expressing Vancomycin Resistance Early after Adhesion to a Metal Surface. BioMed Research International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1057328
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sakimura, Toshiyuki…[et al.]. Biofilm-Forming Staphylococcus epidermidis Expressing Vancomycin Resistance Early after Adhesion to a Metal Surface. BioMed Research International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1057328
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Sakimura, Toshiyuki& Kajiyama, Shiro& Adachi, Shinji& Chiba, Ko& Yonekura, Akihiko& Tomita, Masato…[et al.]. Biofilm-Forming Staphylococcus epidermidis Expressing Vancomycin Resistance Early after Adhesion to a Metal Surface. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1057328
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1057328
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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