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A Novel Reference Plasmid for the Qualitative Detection of Genetically Modified Rice in Food and Feed
المؤلفون المشاركون
Li, Liang
Jin, Wujun
Dong, Mei
An, Na
Liang, Lixia
Wan, Yusong
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-09-30
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Rice is one of the most important food crops in the world.
Genetically modified (GM) technology has been used in rice to confer herbicide tolerance and pathogen or insect resistance.
China invests heavily in research on GM rice.
By the end of 2014, at least 250 transgenic rice lines had been developed in China.
To monitor the presence of GM rice in food and feed, we collected information on foreign elements from 250 transgenic rice lines and found 5 elements, including the Agrobacterium tumefaciens nopaline synthase terminator (T-NOS), the cauliflower mosaic virus 35S promoter (CaMV35S), the ubiquitin gene (Ubi), the bar gene, and the hygromycin phosphotransferase gene (Hpt), that are commonly present in GM rice.
Therefore, we constructed a novel plasmid (pBJGMM001) that contains fragments of these elements and two endogenous reference genes (the sucrose phosphate synthase gene, SPS, and the phosphoenolpyruvate carboxylase gene, PEPC).
pBJGMM001 can serve as a standard for detecting 96% of GM rice lines in China.
The primers, amplicons, reaction mixture, and PCR program were developed based on Chinese National Standards.
The protocol was validated and determined to be suitable for practical use in monitoring and identifying GM rice.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Li, Liang& Dong, Mei& An, Na& Liang, Lixia& Wan, Yusong& Jin, Wujun. 2015. A Novel Reference Plasmid for the Qualitative Detection of Genetically Modified Rice in Food and Feed. BioMed Research International،Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1057345
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Li, Liang…[et al.]. A Novel Reference Plasmid for the Qualitative Detection of Genetically Modified Rice in Food and Feed. BioMed Research International No. 2015 (2015), pp.1-6.
https://search.emarefa.net/detail/BIM-1057345
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Li, Liang& Dong, Mei& An, Na& Liang, Lixia& Wan, Yusong& Jin, Wujun. A Novel Reference Plasmid for the Qualitative Detection of Genetically Modified Rice in Food and Feed. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1057345
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1057345
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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