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ELISA-Based Crossmatching Allowing the Detection of Emerging Donor-Specific Anti-HLA Antibodies through the Use of Stored Donors’ Cell Lysates
المؤلفون المشاركون
Schlaf, G.
Stöhr, K.
Rothhoff, A.
Altermann, W.
المصدر
Case Reports in Transplantation
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-11-08
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
About forty years ago the complement-dependent crossmatch assay (CDC-CM) was developed as standard procedure in order to select recipients without donor-specific antibodies directed against human leukocyte antigens of their given donors since the negative outcome of pretransplant crossmatching represents one of the most important requirements for a successful kidney graft survival.
However, as a functional assay the CDC-CM strongly depends on the availability of donors’ isolated lymphocytes and in particular on their vitality highly limiting its applicability for recipients treated with special drugs and therapeutic antibodies or suffering from underlying autoimmune diseases.
In the great majority of these cases ELISA-based crossmatching has been demonstrated to be an adequate alternative procedure nevertheless leading to valid results.
With these case reports we show for the first time that ELISA-based crossmatching is suitable to demonstrate the upcoming donor-specific anti-HLA antibodies as a consequence of allografting using deep-frozen deceased donor’s material such as blood or spleen detergent lysate.
Thus, this ELISA-based procedure first provides the option to routinely perform crossmatching using stored material of deceased donors in order to substitute or at least to complement virtual crossmatching, that is, the comparison of the recipients’ anti-HLA antibody specificities with the donors’ historically identified HLA types.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Schlaf, G.& Stöhr, K.& Rothhoff, A.& Altermann, W.. 2015. ELISA-Based Crossmatching Allowing the Detection of Emerging Donor-Specific Anti-HLA Antibodies through the Use of Stored Donors’ Cell Lysates. Case Reports in Transplantation،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1060195
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Schlaf, G.…[et al.]. ELISA-Based Crossmatching Allowing the Detection of Emerging Donor-Specific Anti-HLA Antibodies through the Use of Stored Donors’ Cell Lysates. Case Reports in Transplantation No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1060195
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Schlaf, G.& Stöhr, K.& Rothhoff, A.& Altermann, W.. ELISA-Based Crossmatching Allowing the Detection of Emerging Donor-Specific Anti-HLA Antibodies through the Use of Stored Donors’ Cell Lysates. Case Reports in Transplantation. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1060195
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1060195
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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