Toll-Like Receptor 9 Inactivation Alleviated Atherosclerotic Progression and Inhibited Macrophage Polarized to M1 Phenotype in ApoE−− Mice
المؤلفون المشاركون
Lin, Ling
Huang, Ziyang
Ouyang, Qiufang
Chen, Xiaoqing
Ma, Chunmei
Lin, Ye
Hu, Weiping
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-07-15
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Objective.
Toll-like receptor 9 (TLR9) is involved in many inflammatory diseases, but its role in atherosclerosis remains controversial.
This study aimed to investigate the role of TLR9 in atherosclerosis development and macrophage polarization.
Methods.
ApoE−/− mice were treated with vehicle or IRS869 for 12 weeks.
Plaque vulnerability was assessed with immunohistochemical analysis, picro-sirius red, and oil red O staining.
The expressions of M1- and M2-associated markers in plaques were detected by RT-PCR and immunofluorescence.
The aorta TLR9 and its downstream molecules including myeloid differentiation protein 88 (MyD88), phosphorylated nuclear factor-kappa B (p-NF-κB), and interferon regulatory factor 7 (IRF7) were determined by western blot analysis.
The frequency of M1 and M2 subtype in RAW264.7 cells treated with IRS869 and/or ODN1826 was evaluated with flow cytometry.
Results.
In ApoE−/− mice, functional inactivation of TLR9 pathway resulted in attenuated atherosclerosis development, as manifested by reduced plaque burden and by decreased plaque vulnerability.
Mechanistically, TLR9 inhibition prevented the activation of MyD88/NF-κB pathway and shifted the balance of M1/M2 toward M2 macrophages that were involved.
Conclusions.
Our data indicated that TLR9 inactivation ameliorated atherosclerosis via skewing macrophage plasticity to M2 phenotype in ApoE-deficient mice.
These findings may provide a promising therapeutic strategy for atherosclerosis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ma, Chunmei& Ouyang, Qiufang& Huang, Ziyang& Chen, Xiaoqing& Lin, Ye& Hu, Weiping…[et al.]. 2015. Toll-Like Receptor 9 Inactivation Alleviated Atherosclerotic Progression and Inhibited Macrophage Polarized to M1 Phenotype in ApoE−− Mice. Disease Markers،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1061077
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ma, Chunmei…[et al.]. Toll-Like Receptor 9 Inactivation Alleviated Atherosclerotic Progression and Inhibited Macrophage Polarized to M1 Phenotype in ApoE−− Mice. Disease Markers No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1061077
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ma, Chunmei& Ouyang, Qiufang& Huang, Ziyang& Chen, Xiaoqing& Lin, Ye& Hu, Weiping…[et al.]. Toll-Like Receptor 9 Inactivation Alleviated Atherosclerotic Progression and Inhibited Macrophage Polarized to M1 Phenotype in ApoE−− Mice. Disease Markers. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1061077
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1061077
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر