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Panax notoginseng Saponins Attenuate Phenotype Switching of Vascular Smooth Muscle Cells Induced by Notch3 Silencing
المؤلفون المشاركون
Gao, Yonghong
Liu, Nan
Shan, Dazhi
Li, Ying
Chen, Hui
Huang, Yonghua
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-10-11
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Panax notoginseng saponins (PNS) could maintain vascular smooth muscle cells (VSMCs) in stable phenotypes so as to keep blood vessel elasticity as well as prevent failing in endovascular treatment with stent.
Downregulation of Notch3 expression in VSMCs could influence the phenotype of VSMCs under pathologic status.
However, whether PNS is able to attenuate the Notch3 silencing induced phenotype switching of VSMCs remains poorly understood.
Primary human VSMCs were transfected with a plasmid containing a small interfering RNA (siRNA) against Notch3 and then exposed to different doses of PNS.
The control groups included cells not receiving any treatment and cells transfected with a control siRNA.
Phenotypic switching was evaluated by observing cell morphology with confocal microscopy, as well as examining α-SM-actin, SM22α, and OPN using Western blot.
Downregulated Notch3 with a siRNA induced apparent phenotype switching, as reflected by morphologic changes, decreased expression of α-SM-actin and SM22α and increased expression of OPN.
These changes were inhibited by PNS in a dose-dependent manner.
The phenotype switching of VSMCs induced by Notch3 knockdown could be inhibited by PNS in a dose-dependent manner.
Our study provided new evidence for searching effective drug for amending stability of atherosclerotic disease.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Liu, Nan& Shan, Dazhi& Li, Ying& Chen, Hui& Gao, Yonghong& Huang, Yonghua. 2015. Panax notoginseng Saponins Attenuate Phenotype Switching of Vascular Smooth Muscle Cells Induced by Notch3 Silencing. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1061194
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Liu, Nan…[et al.]. Panax notoginseng Saponins Attenuate Phenotype Switching of Vascular Smooth Muscle Cells Induced by Notch3 Silencing. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-6.
https://search.emarefa.net/detail/BIM-1061194
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Liu, Nan& Shan, Dazhi& Li, Ying& Chen, Hui& Gao, Yonghong& Huang, Yonghua. Panax notoginseng Saponins Attenuate Phenotype Switching of Vascular Smooth Muscle Cells Induced by Notch3 Silencing. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1061194
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1061194
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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