Gene Expression Response of Trichophyton rubrum during Coculture on Keratinocytes Exposed to Antifungal Agents
المؤلفون المشاركون
Komoto, Tatiana Takahasi
Bitencourt, Tamires Aparecida
Silva, Gabriel
Beleboni, Rene Oliveira
Marins, Mozart
Fachin, Ana Lúcia
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-07-14
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Trichophyton rubrum is the most common causative agent of dermatomycoses worldwide, causing infection in the stratum corneum, nails, and hair.
Despite the high prevalence of these infections, little is known about the molecular mechanisms involved in the fungal-host interaction, particularly during antifungal treatment.
The aim of this work was to evaluate the gene expression of T.
rubrum cocultured with keratinocytes and treated with the flavonoid trans-chalcone and the glycoalkaloid α-solanine.
Both substances showed a marked antifungal activity against T.
rubrum strain CBS (MIC = 1.15 and 17.8 µg/mL, resp.).
Cytotoxicity assay against HaCaT cells produced IC50 values of 44.18 to trans-chalcone and 61.60 µM to α-solanine.
The interaction of keratinocytes with T.
rubrum conidia upregulated the expression of genes involved in the glyoxylate cycle, ergosterol synthesis, and genes encoding proteases but downregulated the ABC transporter TruMDR2 gene.
However, both antifungals downregulated the ERG1 and ERG11, metalloprotease 4, serine proteinase, and TruMDR2 genes.
Furthermore, the trans-chalcone downregulated the genes involved in the glyoxylate pathway, isocitrate lyase, and citrate synthase.
Considering the urgent need for more efficient and safer antifungals, these results contribute to a better understanding of fungal-host interactions and to the discovery of new antifungal targets.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Komoto, Tatiana Takahasi& Bitencourt, Tamires Aparecida& Silva, Gabriel& Beleboni, Rene Oliveira& Marins, Mozart& Fachin, Ana Lúcia. 2015. Gene Expression Response of Trichophyton rubrum during Coculture on Keratinocytes Exposed to Antifungal Agents. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1061212
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Komoto, Tatiana Takahasi…[et al.]. Gene Expression Response of Trichophyton rubrum during Coculture on Keratinocytes Exposed to Antifungal Agents. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1061212
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Komoto, Tatiana Takahasi& Bitencourt, Tamires Aparecida& Silva, Gabriel& Beleboni, Rene Oliveira& Marins, Mozart& Fachin, Ana Lúcia. Gene Expression Response of Trichophyton rubrum during Coculture on Keratinocytes Exposed to Antifungal Agents. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1061212
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1061212
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر