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Rhein Elicits In Vitro Cytotoxicity in Primary Human Liver HL-7702 Cells by Inducing Apoptosis through Mitochondria-Mediated Pathway
المؤلفون المشاركون
Bounda, Guy-Armel
Zhou, Wang
Wang, Dan-dan
Yu, Feng
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-19، 19ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-06-14
دولة النشر
مصر
عدد الصفحات
19
التخصصات الرئيسية
الملخص EN
Objective.
To study rhein-induced apoptosis signaling pathway and to investigate its molecular mechanisms in primary human hepatic cells.
Results.
Cell viability of HL-7702 cells treated with rhein showed significant decrease in dose-dependent manner.
Following rhein treatment (25 μM, 50 μM, and 100 μM) for 12 h, the detection of apoptotic cells was significantly analyzed by flow cytometry and nuclear morphological changes by Hoechst 33258, respectively.
Fatty degeneration studies showed upregulation level of the relevant hepatic markers (P < 0.01).
Caspase activities expressed significant upregulation of caspase-3, caspase-9, and caspase-8.
Moreover, apoptotic cells by rhein were significantly inhibited by Z-LEHD-FMK and Z-DEVD-FMK, caspase-9 inhibitor, and caspase-3 inhibitor, respectively.
Overproduction of reactive oxygen species, lipid peroxidation, and loss of mitochondrial membrane potential were detected by fluorometry.
Additionally, NAC, a ROS scavenger, significantly attenuated rhein-induced oxidative damage in HL-7702 cells.
Furthermore, real-time qPCR results showed significant upregulation of p53, PUMA, Apaf-1, and Casp-9 and Casp-3 mRNA, with no significant changes of Fas and Cytochrome-c.
Immunoblotting revealed significant Cytochrome-c release from mitochondria into cytosol and no change in Fas expression.
Conclusion.
Taken together, these observations suggested that rhein could induce apoptosis in HL-7702 cells via mitochondria-mediated signal pathway with involvement of oxidative stress mechanism.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Bounda, Guy-Armel& Zhou, Wang& Wang, Dan-dan& Yu, Feng. 2015. Rhein Elicits In Vitro Cytotoxicity in Primary Human Liver HL-7702 Cells by Inducing Apoptosis through Mitochondria-Mediated Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-19.
https://search.emarefa.net/detail/BIM-1061364
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Bounda, Guy-Armel…[et al.]. Rhein Elicits In Vitro Cytotoxicity in Primary Human Liver HL-7702 Cells by Inducing Apoptosis through Mitochondria-Mediated Pathway. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-19.
https://search.emarefa.net/detail/BIM-1061364
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Bounda, Guy-Armel& Zhou, Wang& Wang, Dan-dan& Yu, Feng. Rhein Elicits In Vitro Cytotoxicity in Primary Human Liver HL-7702 Cells by Inducing Apoptosis through Mitochondria-Mediated Pathway. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-19.
https://search.emarefa.net/detail/BIM-1061364
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1061364
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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