Ferulic Acid Attenuates TGF-β1-Induced Renal Cellular Fibrosis in NRK-52E Cells by Inhibiting SmadILKSnail Pathway
المؤلفون المشاركون
Wei, Ming-Gang
Sun, Wei
He, Wei-ming
Ni, Li
Yang, Yan-yu
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-04-08
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Renal fibrosis is a common cause of renal dysfunction with chronic kidney disease.
Central to this process is epithelial-mesenchymal transformation (EMT) of proximal tubular epithelial cells driven by transforming growth factor-β1 (TGF-β1) signaling.
The present study aimed to investigate the effect of Ferulic acid (FA) on EMT of renal proximal tubular epithelial cell line (NRK-52E) induced by TGF-β1 and to elucidate its underlying mechanism against EMT related to TGF-β1/Smads pathway.
The NRK-52E cells were treated for 48 h with TGF-β1 (5 ng/mL) in different concentrations of FA (0 to 200 µM).
Fibronectin, a mesenchymal marker, was assessed by western blotting.
Western blotting was also used to examine the EMT markers (E-cadherin, and α-smooth muscle actin (α-SMA)), signal transducer (p-Smad2/3), and EMT initiator (Snail).
ILK was also assayed by western blotting.
The results showed that TGF-β1 induced spindle-like morphological transition in NRK-52E cells.
Smad2/3 signaling pathway activation, increased fibronectin, α-SMA, ILK, and Snail expression, and decreased E-cadherin expression in TGF-β1-treated NRK-52E cells.
FA efficiently blocked P-Smad2/3 activation and attenuated all these EMT changes induced by TGF-β1.
These findings suggest that FA may serve as a potential fibrosis antagonist for renal proximal tubule cells by inhibiting EMT process.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wei, Ming-Gang& Sun, Wei& He, Wei-ming& Ni, Li& Yang, Yan-yu. 2015. Ferulic Acid Attenuates TGF-β1-Induced Renal Cellular Fibrosis in NRK-52E Cells by Inhibiting SmadILKSnail Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1063389
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wei, Ming-Gang…[et al.]. Ferulic Acid Attenuates TGF-β1-Induced Renal Cellular Fibrosis in NRK-52E Cells by Inhibiting SmadILKSnail Pathway. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1063389
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wei, Ming-Gang& Sun, Wei& He, Wei-ming& Ni, Li& Yang, Yan-yu. Ferulic Acid Attenuates TGF-β1-Induced Renal Cellular Fibrosis in NRK-52E Cells by Inhibiting SmadILKSnail Pathway. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1063389
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1063389
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر