Effects of Berberine on Cell Cycle, DNA, Reactive Oxygen Species, and Apoptosis in L929 Murine Fibroblast Cells
المؤلفون المشاركون
Gu, Manman
Xu, Jing
Han, Chunyang
Kang, Youxi
Liu, Tengfei
He, Yanfei
Huang, Yanfei
Liu, Cuiyan
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-10-13
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Berberine, an isoquinoline alkaloid isolated from several traditional Chinese herbal medicines (TCM), exhibits a strong antimicrobial activity in the treatment of diarrhea.
However, it causes human as well as animal toxicity from heavy dosage.
The present study was conducted to investigate the cytotoxicity of berberine and its possible trigger mechanisms resulting in cell cycle arrest, DNA damage, ROS (reactive oxygen species) level, mitochondrial membrane potential change, and cell apoptosis in L929 murine fibroblast (L929) cells.
The cells were cultured in vitro and treated with different concentrations of berberine for 24 h.
The results showed that cell viability was significantly decreased in a subjected dose-dependent state; berberine concentrations were higher than 0.05 mg/mL.
Berberine at a concentration above 0.1 mg/mL altered the morphology of L929 cells.
Cells at G2/M phase were clear that the level of ROS and cell apoptosis rates increased in 0.1 mg/mL group.
Each DNA damage indicator score (DIS) increased in groups where concentration of berberine was above 0.025 mg/mL.
The mitochondrial membrane potential counteractive balance mechanics were significantly altered when concentrations of berberine were above 0.005 mg/mL.
In all, the present study suggested that berberine at high dosage exhibited cytotoxicity on L929 which was related to resultant: cell cycle arrest; DNA damage; accumulation of intracellular ROS; reduction of mitochondrial membrane potential; and cell apoptosis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Gu, Manman& Xu, Jing& Han, Chunyang& Kang, Youxi& Liu, Tengfei& He, Yanfei…[et al.]. 2015. Effects of Berberine on Cell Cycle, DNA, Reactive Oxygen Species, and Apoptosis in L929 Murine Fibroblast Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1063603
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Gu, Manman…[et al.]. Effects of Berberine on Cell Cycle, DNA, Reactive Oxygen Species, and Apoptosis in L929 Murine Fibroblast Cells. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1063603
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Gu, Manman& Xu, Jing& Han, Chunyang& Kang, Youxi& Liu, Tengfei& He, Yanfei…[et al.]. Effects of Berberine on Cell Cycle, DNA, Reactive Oxygen Species, and Apoptosis in L929 Murine Fibroblast Cells. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1063603
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1063603
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر