Transcriptional Regulation of CYP3A42B62C9 Mediated via Nuclear Receptor PXR by Helicid and Its Metabolites
المؤلفون المشاركون
Li, Yan
Chen, Qun
Xie, Hai-tang
Wang, Guo
Xu, Zhe
Pu, Zhi-chen
Hu, Hua
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-04-22
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Objective.
This study aims at establishing and validating an in vitro system to screen drug inducers of CYPs mediated via hPXR, as well as studying transcriptional regulation of CYPs mediated via hPXR by helicid and its two metabolites.
Methods.
Cloning the nuclear receptor hPXR and the promoters of CYP3A4, CYP2B6, CYP2C9, and inserting the trans-element to the upstream of firefly luciferase reporter gene of the pGL4.17 vectors, then cotransfecting the report vectors and hPXR expression plasmid to HepG2 cell line.
After 24 hours, the transfected cells were treated with helicid (0.004, 0.04, and 0.4 μmol/L) and its metabolite I and metabolite II (0.0004, 0.004, and 0.04 μmol/L) for 48 h, while rifampin (10 μmol/L) was included as the positive control and 0.1% DMSO as the negative control group.
Cells were lysized and luciferase activity was determined using a dual luciferase reporter assay kit.
Results.
Helicid and its metabolites did not significantly increase promoter activities of CYP3A4, CYP2B6, and CYP2C9 in HepG2 cells transfected with PXR expression plasmid (P>0.05).
Conclusion.
PXR-expressed CYP3A4, CYP2B6, and CYP2C9 dual luciferase reporter gene platforms were successfully established, and helicid and its metabolites I, II do not significantly induce the transcription of CYP3A4, CYP2B6, and CYP2C9.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chen, Qun& Xie, Hai-tang& Li, Yan& Wang, Guo& Xu, Zhe& Pu, Zhi-chen…[et al.]. 2015. Transcriptional Regulation of CYP3A42B62C9 Mediated via Nuclear Receptor PXR by Helicid and Its Metabolites. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1063604
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chen, Qun…[et al.]. Transcriptional Regulation of CYP3A42B62C9 Mediated via Nuclear Receptor PXR by Helicid and Its Metabolites. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1063604
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chen, Qun& Xie, Hai-tang& Li, Yan& Wang, Guo& Xu, Zhe& Pu, Zhi-chen…[et al.]. Transcriptional Regulation of CYP3A42B62C9 Mediated via Nuclear Receptor PXR by Helicid and Its Metabolites. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1063604
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1063604
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر